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PMID:20228247

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Citation

Graf, P, Dolzblasz, A, Würschum, T, Lenhard, M, Pfreundt, U and Laux, T (2010) MGOUN1 encodes an Arabidopsis type IB DNA topoisomerase required in stem cell regulation and to maintain developmentally regulated gene silencing. Plant Cell 22:716-28

Abstract

Maintenance of stem cells in the Arabidopsis thaliana shoot meristem is regulated by signals from the underlying cells of the organizing center, provided through the transcription factor WUSCHEL (WUS). Here, we report the isolation of several independent mutants of MGOUN1 (MGO1) as genetic suppressors of ectopic WUS activity and enhancers of stem cell defects in hypomorphic wus alleles. mgo1 mutants have previously been reported to result in a delayed progression of meristem cells into differentiating organ primordia (Laufs et al., 1998). Genetic analyses indicate that MGO1 functions together with WUS in stem cell maintenance at all stages of shoot and floral meristems. Synergistic interactions of mgo1 with several chromatin mutants suggest that MGO1 affects gene expression together with chromatin remodeling pathways. In addition, the expression states of developmentally regulated genes are randomly switched in mgo1 in a mitotically inheritable way, indicating that MGO1 stabilizes epigenetic states against stochastically occurring changes. Positional cloning revealed that MGO1 encodes a putative type IB topoisomerase, which in animals and yeast has been shown to be required for regulation of DNA coiling during transcription and replication. The specific developmental defects in mgo1 mutants link topoisomerase IB function in Arabidopsis to stable propagation of developmentally regulated gene expression.

Links

PubMed PMC2861470 Online version:10.1105/tpc.109.068296

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Chromatin Assembly and Disassembly; Chromosome Mapping; Cloning, Molecular; DNA Topoisomerases, Type I/genetics; DNA Topoisomerases, Type I/metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Gene Silencing; Genes, Plant; Homeodomain Proteins/metabolism; Meristem/cytology; Mutation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4K3F4

acts_upstream_of_or_within

GO:0019827: stem cell population maintenance

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT2G17950

P

Seeded From UniProt

complete

ARATH:F4K3F4

acts_upstream_of_or_within

GO:0006338: chromatin remodeling

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT1G65470

P

Seeded From UniProt

complete

ARATH:F4K3F4

acts_upstream_of_or_within

GO:0009933: meristem structural organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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