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PMID:20212317
| Citation |
Mukhopadhyay, D, Arnaoutov, A and Dasso, M (2010) The SUMO protease SENP6 is essential for inner kinetochore assembly. J. Cell Biol. 188:681-92 |
|---|---|
| Abstract |
We have analyzed the mitotic function of SENP6, a small ubiquitin-like modifier (SUMO) protease that disassembles conjugated SUMO-2/3 chains. Cells lacking SENP6 showed defects in spindle assembly and metaphase chromosome congression. Analysis of kinetochore composition in these cells revealed that a subset of proteins became undetectable on inner kinetochores after SENP6 depletion, particularly the CENP-H/I/K complex, whereas other changes in kinetochore composition mimicked defects previously reported to result from CENP-H/I/K depletion. We further found that CENP-I is degraded through the action of RNF4, a ubiquitin ligase which targets polysumoylated proteins for proteasomal degradation, and that SENP6 stabilizes CENP-I by antagonizing RNF4. Together, these findings reveal a novel mechanism whereby the finely balanced activities of SENP6 and RNF4 control vertebrate kinetochore assembly through SUMO-targeted destabilization of inner plate components. |
| Links |
PubMed PMC2835930 Online version:10.1083/jcb.200909008 |
| Keywords |
Animals; Chromosomes/metabolism; Cysteine Endopeptidases/genetics; Cysteine Endopeptidases/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; HeLa Cells; Histones/genetics; Histones/metabolism; Humans; Kinetochores/metabolism; Kinetochores/ultrastructure; Microtubules/metabolism; Mitosis/physiology; Mitotic Spindle Apparatus/metabolism; Multiprotein Complexes/metabolism; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Protein-Serine-Threonine Kinases/metabolism; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q92674 |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:CENPI |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P78317 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:CENPI |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q9GZR1 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:CENPI |
located_in |
GO:0000776: kinetochore |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:SENP6 |
involved_in |
GO:0070646: protein modification by small protein removal |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:SENP6 |
involved_in |
GO:0090169: regulation of spindle assembly |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:SENP6 |
involved_in |
GO:0090234: regulation of kinetochore assembly |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:SENP6 |
involved_in |
GO:0016926: protein desumoylation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:SENP6 |
enables |
GO:0070139: SUMO-specific endopeptidase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:SENP6 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q92674 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:CENPH |
located_in |
GO:0000776: kinetochore |
ECO:0000315: mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0043161: proteasome-mediated ubiquitin-dependent protein catabolic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0090169: regulation of spindle assembly |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0090234: regulation of kinetochore assembly |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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