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PMID:20194926

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Citation

Liu, D, Gong, Q, Ma, Y, Li, P, Li, J, Yang, S, Yuan, L, Yu, Y, Pan, D, Xu, F and Wang, NN (2010) cpSecA, a thylakoid protein translocase subunit, is essential for photosynthetic development in Arabidopsis. J. Exp. Bot. 61:1655-69

Abstract

The endosymbiont-derived Sec-dependent protein sorting pathway is essential for protein import into the thylakoid lumen and is important for the proper functioning of the chloroplast. Two loss-of-function mutants of cpSecA, the ATPase subunit of the chloroplast Sec translocation machinery, were analysed in Arabidopsis. The homozygous mutants were albino and seedling lethal under autotrophic conditions and remained dwarf and infertile with an exogenous carbon supply. They were subject to oxidative stress and accumulated superoxide under normal lighting conditions. Electron microscopy revealed that the chloroplast of the mutants had underdeveloped thylakoid structures. Histochemical GUS assay of the AtcpSecA::GUS transgenic plants confirmed that AtcpSecA was expressed in green organs in a light-inducible way. Real-time RT-PCR and microarray analysis revealed repressed transcription of nucleus- and chloroplast- encoded subunits of photosynthetic complexes, and induced transcription of chloroplast protein translocation machinery and mitochondrion-encoded respiratory complexes in the mutants. It is inferred that AtcpSecA plays an essential role in chloroplast biogenesis, the absence of which triggered a retrograde signal, eventually leading to a reprogramming of chloroplast and mitochondrial gene expression.

Links

PubMed Online version:10.1093/jxb/erq033

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/physiology; Arabidopsis/ultrastructure; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Chloroplast Proteins; Electrophoresis, Polyacrylamide Gel; Gene Expression Regulation, Plant/genetics; Gene Expression Regulation, Plant/physiology; Membrane Proteins/genetics; Membrane Proteins/metabolism; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Oligonucleotide Array Sequence Analysis; Oxidative Stress/genetics; Oxidative Stress/physiology; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Genetically Modified/genetics; Plants, Genetically Modified/metabolism; Plants, Genetically Modified/physiology; Plants, Genetically Modified/ultrastructure; Protein Subunits/genetics; Protein Subunits/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Thylakoids/enzymology; Thylakoids/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4JG57

involved_in

GO:0010109: regulation of photosynthesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4JG57

involved_in

GO:0010090: trichome morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4JG57

involved_in

GO:0009658: chloroplast organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4JG57

involved_in

GO:0009646: response to absence of light

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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