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PMID:20156967

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Citation

Duran, JM, Anjard, C, Stefan, C, Loomis, WF and Malhotra, V (2010) Unconventional secretion of Acb1 is mediated by autophagosomes. J. Cell Biol. 188:527-36

Abstract

Starving Dictyostelium discoideum cells secrete AcbA, an acyl coenzyme A-binding protein (ACBP) that lacks a conventional signal sequence for entering the endoplasmic reticulum (ER). Secretion of AcbA in D. discoideum requires the Golgi-associated protein GRASP. In this study, we report that starvation-induced secretion of Acb1, the Saccharomyces cerevisiae ACBP orthologue, also requires GRASP (Grh1). This highlights the conserved function of GRASP in unconventional secretion. Although genes required for ER to Golgi or Golgi to cell surface transport are not required for Acb1 secretion in yeast, this process involves autophagy genes and the plasma membrane t-SNARE, Sso1. Inhibiting transport to vacuoles does not affect Acb1 secretion. In sum, our experiments reveal a unique secretory pathway where autophagosomes containing Acb1 evade fusion with the vacuole to prevent cargo degradation. We propose that these autophagosome intermediates fuse with recycling endosomes instead to form multivesicular body carriers that then fuse with the plasma membrane to release cargo.

Links

PubMed PMC2828925 Online version:10.1083/jcb.200911154

Keywords

Amino Acid Sequence; Autophagy/genetics; Carrier Proteins/chemistry; Carrier Proteins/secretion; Cell Membrane/metabolism; Genes, Fungal/genetics; Golgi Apparatus/metabolism; Membrane Fusion; Models, Biological; Molecular Sequence Data; Multivesicular Bodies/metabolism; Peptides/metabolism; Phagosomes/metabolism; Qa-SNARE Proteins/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/secretion; Secretory Pathway; Vacuoles/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:ACBP

located_in

GO:0005576: extracellular region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:ACBP

located_in

GO:0005576: extracellular region

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:GRH1

involved_in

GO:0009306: protein secretion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(SGD:S000003269)
  • happens_during:(GO:0009267)

Seeded From UniProt

complete

YEAST:BUG1

involved_in

GO:0009306: protein secretion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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