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PMID:20153199

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Citation

Benna, C, Bonaccorsi, S, Wülbeck, C, Helfrich-Förster, C, Gatti, M, Kyriacou, CP, Costa, R and Sandrelli, F (2010) Drosophila timeless2 is required for chromosome stability and circadian photoreception. Curr. Biol. 20:346-52

Abstract

In Drosophila, there are two timeless paralogs, timeless1 (tim1) and timeless2 (tim2, or timeout). Phylogenetic analyses suggest that tim1 originated as a duplication of tim2 around the time of the Cambrian explosion. The function of tim1 as a canonical circadian component is well established, but the role of tim2 in the fly is poorly understood. Many organisms possess a single tim2-like gene that has been implicated in DNA synthesis and, in the case of mammals, somewhat controversially, in circadian rhythmicity. Here we analyze the structure and the functional role of fly tim2. tim2 is a large locus (approximately 75 kb) that harbors several transcribed intronic sequences. Using insertional mutations and tissue-specific RNA interference-mediated downregulation, we find that tim2 is an essential gene required for normal DNA metabolism and chromosome integrity. Moreover, tim2 is involved in light entrainment of the adult circadian clock, via its expression in the T1 basket cells of the optic lobes. tim2's residual role in light entrainment thus provides an evolutionary link that may explain why its derived paralog, tim1, came to play such a major role in both circadian photosensitivity and core clock function.

Links

PubMed Online version:10.1016/j.cub.2009.12.048

Keywords

Analysis of Variance; Animals; Biological Clocks/genetics; Blotting, Northern; Chromosomal Instability/genetics; Circadian Rhythm/genetics; Cytogenetic Analysis; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila Proteins/physiology; Drosophila melanogaster/genetics; Gene Components; Gene Expression Profiling; Gene Expression Regulation, Developmental/genetics; Genes, Essential/genetics; In Situ Hybridization; Light; Optic Lobe, Nonmammalian/metabolism; RNA Interference; Reverse Transcriptase Polymerase Chain Reaction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q8INH7

involved_in

GO:0009649: entrainment of circadian clock

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8INH7

involved_in

GO:0000077: DNA damage checkpoint

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9NAX2

involved_in

GO:0009649: entrainment of circadian clock

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9NAX2

involved_in

GO:0000077: DNA damage checkpoint

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9NG27

involved_in

GO:0009649: entrainment of circadian clock

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9NG27

involved_in

GO:0000077: DNA damage checkpoint

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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