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PMID:20150280

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Citation

Pines, MK, Housden, BE, Bernard, F, Bray, SJ and Röper, K (2010) The cytolinker Pigs is a direct target and a negative regulator of Notch signalling. Development 137:913-22

Abstract

Gas2-like proteins harbour putative binding sites for both the actin and the microtubule cytoskeleton and could thus mediate crosstalk between these cytoskeletal systems. Family members are highly conserved in all metazoans but their in vivo role is not clear. The sole Drosophila Gas2-like gene, CG3973 (pigs), was recently identified as a transcriptional target of Notch signalling and might therefore link cell fate decisions through Notch activation directly to morphogenetic changes. We have generated a null mutant in CG3973 (pigs): pigs(1) mutants are semi-viable but adult flies are flightless, showing indirect flight muscle degeneration, and females are sterile, showing disrupted oogenesis and severe defects in follicle cell differentiation, similar to phenotypes seen when levels of Notch/Delta signalling are perturbed in these tissues. Loss of Pigs leads to an increase in Notch signalling activity in several tissues. These results indicate that Gas2-like proteins are essential for development and suggest that Pigs acts downstream of Notch as a morphogenetic read-out, and also as part of a regulatory feedback loop to relay back information about the morphogenetic state of cells to restrict Notch activation to appropriate levels in certain target tissues.

Links

PubMed PMC2834460 Online version:10.1242/dev.043224

Keywords

Acyltransferases/genetics; Acyltransferases/metabolism; Acyltransferases/physiology; Animals; Cell Differentiation/genetics; Cell Shape/genetics; Drosophila/genetics; Drosophila/growth & development; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Female; Gene Expression Regulation; Growth and Development/genetics; Intracellular Signaling Peptides and Proteins/genetics; Intracellular Signaling Peptides and Proteins/physiology; Male; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Microfilament Proteins/physiology; Models, Biological; Morphogenesis/genetics; Ovarian Follicle/cytology; Ovarian Follicle/growth & development; Ovarian Follicle/metabolism; Plakins/genetics; Plakins/metabolism; Plakins/physiology; Receptors, Notch/genetics; Receptors, Notch/metabolism; Receptors, Notch/physiology; Sequence Homology; Signal Transduction/genetics; Signal Transduction/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:GA2PE

involved_in

GO:0030713: ovarian follicle cell stalk formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:GA2PE

involved_in

GO:0030707: ovarian follicle cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:GA2PE

colocalizes_with

GO:0015629: actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:GA2PE

colocalizes_with

GO:0005881: cytoplasmic microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:GA2PE

involved_in

GO:0045746: negative regulation of Notch signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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