GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20133881

From GONUTS
Jump to: navigation, search
Citation

Kuromori, T, Miyaji, T, Yabuuchi, H, Shimizu, H, Sugimoto, E, Kamiya, A, Moriyama, Y and Shinozaki, K (2010) ABC transporter AtABCG25 is involved in abscisic acid transport and responses. Proc. Natl. Acad. Sci. U.S.A. 107:2361-6

Abstract

Abscisic acid (ABA) is one of the most important phytohormones involved in abiotic stress responses, seed maturation, germination, and senescence. ABA is predominantly produced in vascular tissues and exerts hormonal responses in various cells, including guard cells. Although ABA responses require extrusion of ABA from ABA-producing cells in an intercellular ABA signaling pathway, the transport mechanisms of ABA through the plasma membrane remain unknown. Here we isolated an ATP-binding cassette (ABC) transporter gene, AtABCG25, from Arabidopsis by genetically screening for ABA sensitivity. AtABCG25 was expressed mainly in vascular tissues. The fluorescent protein-fused AtABCG25 was localized at the plasma membrane in plant cells. In membrane vesicles derived from AtABCG25-expressing insect cells, AtABCG25 exhibited ATP-dependent ABA transport. The AtABCG25-overexpressing plants showed higher leaf temperatures, implying an influence on stomatal regulation. These results strongly suggest that AtABCG25 is an exporter of ABA and is involved in the intercellular ABA signaling pathway. The presence of the ABA transport mechanism sheds light on the active control of multicellular ABA responses to environmental stresses among plant cells.

Links

PubMed PMC2836683 Online version:10.1073/pnas.0912516107

Keywords

ATP-Binding Cassette Transporters/genetics; ATP-Binding Cassette Transporters/metabolism; Abscisic Acid/metabolism; Animals; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Base Sequence; Cell Line; Cell Membrane/metabolism; DNA Primers/genetics; Gene Expression; Genes, Plant; Mutation; Plant Growth Regulators/metabolism; Plants, Genetically Modified; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Spodoptera; Tissue Distribution; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:AB25G

enables

GO:0015562: efflux transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:AB25G

involved_in

GO:0010496: intercellular transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AB25G

involved_in

GO:0009738: abscisic acid-activated signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AB25G

involved_in

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AB25G

involved_in

GO:0140352: export from cell

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AB25G

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:AB25G

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AB25G

enables

GO:0042626: ATPase-coupled transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:AB25G

acts_upstream_of_or_within

GO:0080168: abscisic acid transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.