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PMID:20118605

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Citation

Chahomchuen, T, Hondo, K, Ohsaki, M, Sekito, T and Kakinuma, Y (2009) Evidence for Avt6 as a vacuolar exporter of acidic amino acids in Saccharomyces cerevisiae cells. J. Gen. Appl. Microbiol. 55:409-17

Abstract

Here we examined the significance of Avt6, a vacuolar exporter of glutamate and aspartate suggested by the in vitro membrane vesicle experiment, in vacuolar compartmentalization of amino acids in Saccharomyces cerevisiae cells. Fluorescent microscopic observation of GFP-fused Avt6 revealed it to be exclusively localized to the vacuolar membrane, with the amount of Myc-tagged Avt6 significantly increased under nitrogen starvation. Glutamate uptake by cells was enhanced by deletion of the AVT6 gene, indicating indirect involvement of Avt6 in cellular glutamate accumulation. Differences in acidic amino acid content of both total and vacuolar fractions were insignificant between the parent and avt6Delta cells when cultured in nutrient-rich conditions. However, in nitrogen-starved conditions, the amount of glutamate and aspartate in the vacuolar fraction was notably increased in the avt6Delta cells. Avt6 is thus involved in vacuolar amino acid compartmentalization in S. cerevisiae cells, especially under conditions of nitrogen starvation.

Links

PubMed

Keywords

Amino Acid Transport Systems/metabolism; Amino Acids, Acidic/metabolism; Aspartic Acid/metabolism; Biological Transport; Culture Media; Gene Expression Regulation, Fungal; Glutamic Acid/metabolism; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Intracellular Membranes/metabolism; Nitrogen/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Vacuoles/metabolism

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

YEAST:AVT6

GO:0000329

fungal-type vacuole membrane

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

SGD:AVT6

GO:0000329

fungal-type vacuole membrane

IDA: Inferred from Direct Assay

C

From SGD



See also

References

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