GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

It is now the 4th OPEN Period for CACAO Spring 2019! It will end on Sunday March 24, 2019 at 11:59 pm CDT
This is your chance to make annotations OR challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.
Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search

Chahomchuen, T, Hondo, K, Ohsaki, M, Sekito, T and Kakinuma, Y (2009) Evidence for Avt6 as a vacuolar exporter of acidic amino acids in Saccharomyces cerevisiae cells. J. Gen. Appl. Microbiol. 55:409-17


Here we examined the significance of Avt6, a vacuolar exporter of glutamate and aspartate suggested by the in vitro membrane vesicle experiment, in vacuolar compartmentalization of amino acids in Saccharomyces cerevisiae cells. Fluorescent microscopic observation of GFP-fused Avt6 revealed it to be exclusively localized to the vacuolar membrane, with the amount of Myc-tagged Avt6 significantly increased under nitrogen starvation. Glutamate uptake by cells was enhanced by deletion of the AVT6 gene, indicating indirect involvement of Avt6 in cellular glutamate accumulation. Differences in acidic amino acid content of both total and vacuolar fractions were insignificant between the parent and avt6Delta cells when cultured in nutrient-rich conditions. However, in nitrogen-starved conditions, the amount of glutamate and aspartate in the vacuolar fraction was notably increased in the avt6Delta cells. Avt6 is thus involved in vacuolar amino acid compartmentalization in S. cerevisiae cells, especially under conditions of nitrogen starvation.




Amino Acid Transport Systems/metabolism; Amino Acids, Acidic/metabolism; Aspartic Acid/metabolism; Biological Transport; Culture Media; Gene Expression Regulation, Fungal; Glutamic Acid/metabolism; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Intracellular Membranes/metabolism; Nitrogen/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Vacuoles/metabolism



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status



GO:0000329: fungal-type vacuole membrane

ECO:0000314: direct assay evidence used in manual assertion


Seeded From UniProt



GO:0000329: fungal-type vacuole membrane

IDA: Inferred from Direct Assay:


From SGD

See also


See Help:References for how to manage references in GONUTS.