GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20116245

From GONUTS
Jump to: navigation, search
Citation

Bembenek, JN, White, JG and Zheng, Y (2010) A role for separase in the regulation of RAB-11-positive vesicles at the cleavage furrow and midbody. Curr. Biol. 20:259-64

Abstract

Cell division requires coordinated regulation of chromosome segregation and cytokinesis. Although much is known about the function of the protease separase in promoting sister chromosome separation, the role of separase during cytokinesis is unclear. We show that separase localizes to the ingressing furrow and midbody during cytokinesis in the C. elegans embryo. Loss of separase function during the early mitotic divisions causes cytokinesis failure that is not due to eggshell defects or chromosome nondisjunction. Moreover, depletion of separase causes the accumulation of RAB-11-positive vesicles at the cleavage furrow and midbody that is not a consequence of chromosome nondisjunction, but is mimicked by depletion of vesicle fusion machinery. Collectively, these data indicate that separase is required for cytokinesis by regulating the incorporation of RAB-11-positive vesicles into the plasma membrane at the cleavage furrow and midbody.

Links

PubMed PMC2833016 Online version:10.1016/j.cub.2009.12.045

Keywords

Animals; Animals, Genetically Modified; Caenorhabditis elegans/cytology; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Chromosome Segregation/physiology; Cytokinesis/physiology; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; rab GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:SEP1

located_in

GO:0005813: centrosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0070090: metaphase plate

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0005819: spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0000794: condensed nuclear chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0032154: cleavage furrow

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0030496: midbody

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0051306: mitotic sister chromatid separation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0017157: regulation of exocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0000281: mitotic cytokinesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:STX4

involved_in

GO:0017157: regulation of exocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:TOP2

involved_in

GO:0051306: mitotic sister chromatid separation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:STX4

involved_in

GO:0017157: regulation of exocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

happens_during:(GO:0000281)

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.