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PMID:20113437

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Citation

Balazadeh, S, Siddiqui, H, Allu, AD, Matallana-Ramirez, LP, Caldana, C, Mehrnia, M, Zanor, MI, Köhler, B and Mueller-Roeber, B (2010) A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence. Plant J. 62:250-64

Abstract

The onset and progression of senescence are under genetic and environmental control. The Arabidopsis thaliana NAC transcription factor ANAC092 (also called AtNAC2 and ORE1) has recently been shown to control age-dependent senescence, but its mode of action has not been analysed yet. To explore the regulatory network administered by ANAC092 we performed microarray-based expression profiling using estradiol-inducible ANAC092 overexpression lines. Approximately 46% of the 170 genes up-regulated upon ANAC092 induction are known senescence-associated genes, suggesting that the NAC factor exerts its role in senescence through a regulatory network that includes many of the genes previously reported to be senescence regulated. We selected 39 candidate genes and confirmed their time-dependent response to enhanced ANAC092 expression by quantitative RT-PCR. We also found that the majority of them (24 genes) are up-regulated by salt stress, a major promoter of plant senescence, in a manner similar to that of ANAC092, which itself is salt responsive. Furthermore, 24 genes like ANAC092 turned out to be stage-dependently expressed during seed growth with low expression at early and elevated expression at late stages of seed development. Disruption of ANAC092 increased the rate of seed germination under saline conditions, whereas the opposite occurred in respective overexpression plants. We also detected a delay of salinity-induced chlorophyll loss in detached anac092-1 mutant leaves. Promoter-reporter (GUS) studies revealed transcriptional control of ANAC092 expression during leaf and flower ageing and in response to salt stress. We conclude that ANAC092 exerts its functions during senescence and seed germination through partly overlapping target gene sets.

Links

PubMed Online version:10.1111/j.1365-313X.2010.04151.x

Keywords

Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cluster Analysis; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Regulatory Networks; Germination; Oligonucleotide Array Sequence Analysis; Plants, Genetically Modified/drug effects; Plants, Genetically Modified/genetics; Plants, Genetically Modified/growth & development; Promoter Regions, Genetic; RNA, Plant/genetics; Repressor Proteins/genetics; Repressor Proteins/metabolism; Seeds/growth & development; Sodium Chloride/pharmacology; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:NAC92

involved_in

GO:0010029: regulation of seed germination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NAC92

involved_in

GO:1900057: positive regulation of leaf senescence

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NAC92

acts_upstream_of_or_within

GO:0010468: regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NAC92

acts_upstream_of_or_within

GO:0010150: leaf senescence

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NAC92

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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