GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20112046

From GONUTS
Jump to: navigation, search
Citation

Yang, Q, Gong, ZJ, Zhou, Y, Yuan, JQ, Cheng, J, Tian, L, Li, S, Lin, XD, Xu, R, Zhu, ZR and Mao, C (2010) Role of Drosophila alkaline ceramidase (Dacer) in Drosophila development and longevity. Cell. Mol. Life Sci. 67:1477-90

Abstract

Ceramidases catalyze the hydrolysis of ceramides to generate sphingosine (SPH) and fatty acids, and ceramide metabolism is implicated in various biological responses in Drosophila melanogaster. Here we report the cloning, biochemical characterization, and functional analysis of a Drosophila alkaline ceramidase (Dacer). Dacer, a membrane-bound protein of 284 amino acids, shares homology with yeast and mammalian alkaline ceramidases. Overexpression of Dacer in High Five insect cells increases ceramidase activity in the alkaline pH range, indicating that Dacer is a bona fide alkaline ceramidase. Dacer mRNA is highly expressed in the midgut and at the pupal stage. An inactivation of Dacer by insertional mutagenesis increases the levels of ceramides in both Drosophila pupae and adult flies. Dacer inactivation increases Drosophila pre-adult development time, lifespan, and anti-oxidative stress capacity. Collectively, these results suggest that Dacer plays an important role in the Drosophila development and longevity by controlling the metabolism of ceramides.

Links

PubMed Online version:10.1007/s00018-010-0260-7

Keywords

Alkaline Ceramidase/genetics; Alkaline Ceramidase/metabolism; Amino Acid Sequence; Animals; Base Sequence; Cell Line; Ceramides/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/enzymology; Drosophila melanogaster/growth & development; Drosophila melanogaster/physiology; Female; Humans; Longevity/physiology; Male; Methyltransferases/genetics; Methyltransferases/metabolism; Molecular Sequence Data; Mutagenesis, Insertional; Oxidative Stress; Pupa/anatomy & histology; Pupa/metabolism; Reactive Oxygen Species/metabolism; Sequence Homology, Amino Acid; Spectrometry, Mass, Electrospray Ionization; Sphingosine/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:ACASE

enables

GO:0017040: N-acylsphingosine amidohydrolase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:ACASE

involved_in

GO:0046514: ceramide catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.