GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20097871

From GONUTS
Jump to: navigation, search
Citation

Chen, M and Thelen, JJ (2010) The plastid isoform of triose phosphate isomerase is required for the postgerminative transition from heterotrophic to autotrophic growth in Arabidopsis. Plant Cell 22:77-90

Abstract

During postgerminative seedling establishment, reserves stored during seed filling are mobilized to provide energy and carbon for the growing seedling until autotrophic growth is possible. A plastid isoform of triose phosphate isomerase (pdTPI) plays a crucial role in this transition from heterotrophic to autotrophic growth. A T-DNA insertion in Arabidopsis thaliana pdTPI resulted in a fivefold reduction in transcript, reduced TPI activity, and a severely stunted and chlorotic seedling that accumulated dihydroxyacetone phosphate (DHAP), glycerol, and glycerol-3-phosphate. Methylglyoxal (MG), a by-product of DHAP, also accumulated in the pdtpi mutant. Wild-type seed sown in the presence of any of these four metabolites resulted in a phenocopy of this pdtpi mutant, although MG and DHAP were the most effective based upon dosage. These metabolites (except MG) are by-products of triacylglycerol mobilization and precursors for glycerolipid synthesis, suggesting that lipid metabolism may also be affected. Lipid profiling revealed lower monogalactosyl but higher digalactosyl lipids. It is unclear whether the change in lipid composition is a direct or indirect consequence of the pdtpi mutation, as ribulose-1,5-bis-phosphate carboxylase/oxygenase expression, chloroplast morphology, and starch synthesis are also defective in this mutant. We propose that DHAP and MG accumulation in developing plastids delays the transition from heterotrophic to autotrophic growth, possibly due to MG toxicity.

Links

PubMed PMC2828694 Online version:10.1105/tpc.109.071837

Keywords

Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Autotrophic Processes; Chloroplasts/ultrastructure; DNA, Bacterial/genetics; Dihydroxyacetone Phosphate/metabolism; Gene Expression Regulation, Plant; Germination; Glycerol/metabolism; Glycerophosphates/metabolism; Heterotrophic Processes; Isoenzymes/genetics; Isoenzymes/metabolism; Lipid Metabolism; Mutagenesis, Insertional; Mutation; Plants, Genetically Modified/enzymology; Plants, Genetically Modified/genetics; Plants, Genetically Modified/growth & development; Pyruvaldehyde/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Triose-Phosphate Isomerase/genetics; Triose-Phosphate Isomerase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:TPIC

involved_in

GO:0019563: glycerol catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TPIC

involved_in

GO:0032504: multicellular organism reproduction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TPIC

involved_in

GO:0006642: triglyceride mobilization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TPIC

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TPIC

involved_in

GO:0009658: chloroplast organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TPIC

enables

GO:0004807: triose-phosphate isomerase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:TPIC

involved_in

GO:0046166: glyceraldehyde-3-phosphate biosynthetic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TPIC

involved_in

GO:0080022: primary root development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.