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PMID:20096681

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Citation

Christie, TL, Carter, A, Rollins, EL and Childs, SJ (2010) Syk and Zap-70 function redundantly to promote angioblast migration. Dev. Biol. 340:22-9

Abstract

Spleen tyrosine kinase (Syk) plays critical roles in B-cell and T-cell development, the maintenance of vascular integrity, and proper partitioning of the blood vascular and lymphatic vascular system. Here, we utilize the zebrafish as an in vivo system to demonstrate novel roles for Syk and the related kinase Zeta associated protein (Zap-70) in promoting angioblast migration. Partial knockdown of either gene results in early angiogenic delay of the intersegmental vessels, dorsal intersegmental vessel patterning defects, and partial loss of the thoracic duct. Higher dose knockdown of both genes results in little to no angiogenic sprouting of the intersegmental vessels, a phenotype which resembles knockdown of vegfa. Di-phosphorylated ERK, an effector of the vegfa pathway, is also downregulated in the aorta of syk:zap double morphants. Over-expression of syk under the control of a blood-specific or vascular-specific promoter rescues sprouting defects after loss of vegfa. Together these results suggest that syk and zap-70 function redundantly in an early progenitor to promote the migration of intersegmental vessel angioblasts and lymphangioblasts that contribute to the thoracic duct, either downstream of, or in parallel to vegfa.

Links

PubMed Online version:10.1016/j.ydbio.2010.01.011

Keywords

Animals; B-Lymphocytes/metabolism; Blood Vessels/cytology; Body Patterning/physiology; Cell Movement/physiology; Embryo, Nonmammalian/metabolism; Flow Cytometry; Intracellular Signaling Peptides and Proteins/genetics; Intracellular Signaling Peptides and Proteins/metabolism; Phylogeny; Protein-Tyrosine Kinases/genetics; Protein-Tyrosine Kinases/metabolism; Stem Cells/physiology; T-Lymphocytes/metabolism; Thoracic Duct/cytology; ZAP-70 Protein-Tyrosine Kinase/genetics; ZAP-70 Protein-Tyrosine Kinase/metabolism; Zebrafish/embryology; Zebrafish/metabolism; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:E9QFU7

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-1

P

Seeded From UniProt

complete

DANRE:E9QFU7

involved_in

GO:0001946: lymphangiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:E9QFU7

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-2

P

Seeded From UniProt

complete

DANRE:E9QFU7

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-1

P

Seeded From UniProt

complete

DANRE:E9QFU7

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:F1QFN4

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-1

P

Seeded From UniProt

complete

DANRE:F1QFN4

involved_in

GO:0001946: lymphangiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:F1QFN4

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-2

P

Seeded From UniProt

complete

DANRE:F1QFN4

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-1

P

Seeded From UniProt

complete

DANRE:F1QFN4

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:M9MM92

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-4

P

Seeded From UniProt

complete

DANRE:M9MM92

involved_in

GO:0001946: lymphangiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:M9MM92

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-4

P

Seeded From UniProt

complete

DANRE:M9MM92

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-3

P

Seeded From UniProt

complete

DANRE:M9MM92

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:M9MMB1

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-4

P

Seeded From UniProt

complete

DANRE:M9MMB1

involved_in

GO:0001946: lymphangiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:M9MMB1

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-4

P

Seeded From UniProt

complete

DANRE:M9MMB1

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-3

P

Seeded From UniProt

complete

DANRE:M9MMB1

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:VGFAA

involved_in

GO:0001525: angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-051128-8

P

Seeded From UniProt

complete

DANRE:Q503P3

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-4

P

Seeded From UniProt

complete

DANRE:Q503P3

involved_in

GO:0001946: lymphangiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:Q503P3

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-4

P

Seeded From UniProt

complete

DANRE:Q503P3

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-3

P

Seeded From UniProt

complete

DANRE:Q503P3

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:Q910A2

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-1

P

Seeded From UniProt

complete

DANRE:Q910A2

involved_in

GO:0001946: lymphangiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete

DANRE:Q910A2

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-2

P

Seeded From UniProt

complete

DANRE:Q910A2

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100407-1

P

Seeded From UniProt

complete

DANRE:Q910A2

involved_in

GO:0090050: positive regulation of cell migration involved in sprouting angiogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-040702-3,ZFIN:ZDB-GENE-050522-257

P

Seeded From UniProt

complete


See also

References

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