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PMID:20079334

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Citation

Kumar, SV and Wigge, PA (2010) H2A.Z-containing nucleosomes mediate the thermosensory response in Arabidopsis. Cell 140:136-47

Abstract

Plants are highly sensitive to temperature and can perceive a difference of as little as 1 degrees C. How temperature is sensed and integrated in development is unknown. In a forward genetic screen in Arabidopsis, we have found that nucleosomes containing the alternative histone H2A.Z are essential to perceiving ambient temperature correctly. Genotypes deficient in incorporating H2A.Z into nucleosomes phenocopy warm grown plants, and show a striking constitutive warm temperature transcriptome. We show that nucleosomes containing H2A.Z display distinct responses to temperature in vivo, independently of transcription. Using purified nucleosomes, we are able to show that H2A.Z confers distinct DNA-unwrapping properties on nucleosomes, indicating a direct mechanism for the perception of temperature through DNA-nucleosome fluctuations. Our results show that H2A.Z-containing nucleosomes provide thermosensory information that is used to coordinate the ambient temperature transcriptome. We observe the same effect in budding yeast, indicating that this is an evolutionarily conserved mechanism.

Links

PubMed Online version:10.1016/j.cell.2009.11.006

Keywords

Arabidopsis/physiology; Arabidopsis Proteins/metabolism; DNA, Plant/metabolism; Gene Expression Profiling; HSP70 Heat-Shock Proteins/metabolism; Histones/physiology; Microfilament Proteins/metabolism; Nucleosomes/metabolism; Temperature

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:H2AV1

acts_upstream_of_or_within

GO:0016048: detection of temperature stimulus

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:At1g52740

P

Seeded From UniProt

complete

ARATH:ARP6

acts_upstream_of_or_within

GO:0006355: regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ARP6

acts_upstream_of_or_within

GO:0006338: chromatin remodeling

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ARP6

acts_upstream_of_or_within

GO:0009266: response to temperature stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:H2AV3

acts_upstream_of_or_within

GO:0009266: response to temperature stimulus

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:At3g54560

P

Seeded From UniProt

complete

ARATH:HS704

acts_upstream_of_or_within

GO:0009266: response to temperature stimulus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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