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PMID:20071466

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Citation

Tang, L, Machacek, T, Mamnun, YM, Penkner, A, Gloggnitzer, J, Wegrostek, C, Konrat, R, Jantsch, MF, Loidl, J and Jantsch, V (2010) Mutations in Caenorhabditis elegans him-19 show meiotic defects that worsen with age. Mol. Biol. Cell 21:885-96

Abstract

From a screen for meiotic Caenorhabditis elegans mutants based on high incidence of males, we identified a novel gene, him-19, with multiple functions in prophase of meiosis I. Mutant him-19(jf6) animals show a reduction in pairing of homologous chromosomes and subsequent bivalent formation. Consistently, synaptonemal complex formation is spatially restricted and possibly involves nonhomologous chromosomes. Also, foci of the recombination protein RAD-51 occur delayed or cease altogether. Ultimately, mutation of him-19 leads to chromosome missegregation and reduced offspring viability. The observed defects suggest that HIM-19 is important for both homology recognition and formation of meiotic DNA double-strand breaks. It therefore seems to be engaged in an early meiotic event, resembling in this respect the regulator kinase CHK-2. Most astonishingly, him-19(jf6) hermaphrodites display worsening of phenotypes with increasing age, whereas defects are more severe in female than in male meiosis. This finding is consistent with depletion of a him-19-dependent factor during the production of oocytes. Further characterization of him-19 could contribute to our understanding of age-dependent meiotic defects in humans.

Links

PubMed PMC2836969 Online version:10.1091/mbc.E09-09-0811

Keywords

Amino Acid Sequence; Animals; Caenorhabditis elegans/anatomy & histology; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Chromosome Pairing/genetics; DNA Breaks, Double-Stranded; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Female; Gonads/anatomy & histology; Humans; Male; Meiosis/physiology; Molecular Sequence Data; Mutation; Oogenesis/physiology; Phenotype; RNA Splicing; Recombination, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:Q9N2V7

involved_in

GO:0090173: regulation of synaptonemal complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00278479

P

Seeded From UniProt

complete

CAEEL:Q9N2V7

involved_in

GO:0042138: meiotic DNA double-strand break formation

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00278479

P

Seeded From UniProt

complete


See also

References

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