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PMID:20070309

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Citation

Tung, SM, Unal, C, Ley, A, Peña, C, Tunggal, B, Noegel, AA, Krut, O, Steinert, M and Eichinger, L (2010) Loss of Dictyostelium ATG9 results in a pleiotropic phenotype affecting growth, development, phagocytosis and clearance and replication of Legionella pneumophila. Cell. Microbiol. 12:765-80

Abstract

Infection of Dictyostelium discoideum with Legionella pneumophila resulted in a large number of differentially regulated genes among them three core autophagy genes, ATG8, ATG9 and ATG16. Macroautophagy contributes to many physiological and pathological processes and might also constitute an important mechanism in cell-autonomous immunity. For further studies we selected the highly conserved ATG9. In colocalization studies with GFP-tagged ATG9 and different organelle marker proteins we neither observed colocalization with mitochondria, the ER nor lysosomes. However, there was partial colocalization with the Golgi apparatus and many ATG9-GFP-containing vesicles localized along microtubules and accumulated around the microtubule organizing centre. ATG9-deficient cells had pleiotropic defects. In addition to growth defects they displayed severe developmental defects, consistent with the known role of autophagy in Dictyostelium development. Unexpectedly, the ATG9 mutant also had a strong phagocytosis defect that was particularly apparent when infecting the cells with L. pneumophila. However, those Legionellae that entered the host could multiply better in mutant than in wild-type cells, because of a less efficient clearance in the early and a more efficient replication in the late phase of infection. We conclude that ATG9 and hence macroautophagy has a protective role during pathogen infection.

Links

PubMed Online version:10.1111/j.1462-5822.2010.01432.x

Keywords

Dictyostelium/genetics; Dictyostelium/growth & development; Dictyostelium/immunology; Dictyostelium/microbiology; Gene Knockout Techniques; Golgi Apparatus/chemistry; Legionella pneumophila/growth & development; Microtubules/chemistry; Phagocytosis; Protozoan Proteins/analysis; Protozoan Proteins/genetics; Protozoan Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:ATG9

located_in

GO:0005815: microtubule organizing center

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:ATG9

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:ATG9

located_in

GO:0005874: microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:ATG9

acts_upstream_of_or_within

GO:0019954: asexual reproduction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:ATG9

acts_upstream_of_or_within

GO:0009617: response to bacterium

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:ATG9

acts_upstream_of_or_within

GO:0031152: aggregation involved in sorocarp development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:ATG9

acts_upstream_of_or_within

GO:0031153: slug development involved in sorocarp development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:ATG9

acts_upstream_of_or_within

GO:0031288: sorocarp morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:ATG9

acts_upstream_of_or_within

GO:0006909: phagocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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