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PMID:20053799

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Citation

Rietz, S, Dermendjiev, G, Oppermann, E, Tafesse, FG, Effendi, Y, Holk, A, Parker, JE, Teige, M and Scherer, GF (2010) Roles of Arabidopsis patatin-related phospholipases a in root development are related to auxin responses and phosphate deficiency. Mol Plant 3:524-38

Abstract

Phospholipase A enzymes cleave phospho- and galactolipids to generate free fatty acids and lysolipids that function in animal and plant hormone signaling. Here, we describe three Arabidopsis patatin-related phospholipase A (pPLA) genes AtPLAIVA, AtPLAIVB, and AtPLAIVC and their corresponding proteins. Loss-of-function mutants reveal roles for these pPLAs in roots during normal development and under phosphate deprivation. AtPLAIVA is expressed strongly and exclusively in roots and AtplaIVA-null mutants have reduced lateral root development, characteristic of an impaired auxin response. By contrast, AtPLAIVB is expressed weakly in roots, cotyledons, and leaves but is transcriptionally induced by auxin, although AtplaIVB mutants develop normally. AtPLAIVC is expressed in the floral gynaecium and is induced by abscisic acid (ABA) or phosphate deficiency in roots. While an AtplaIVC-1 loss-of-function mutant displays ABA responsiveness, it exhibits an impaired response to phosphate deficiency during root development. Recombinant AtPLA proteins hydrolyze preferentially galactolipids and, less efficiently, phospholipids, although these enzymes are not localized in chloroplasts. We find that AtPLAIVA and AtPLAIVB are phosphorylated by calcium-dependent protein kinases in vitro and this enhances their activities on phosphatidylcholine but not on phosphatidylglycerol. Taken together, the data reveal novel functions of pPLAs in root development with individual roles at the interface between phosphate deficiency and auxin signaling.

Links

PubMed Online version:10.1093/mp/ssp109

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Indoleacetic Acids/metabolism; Molecular Sequence Data; Phosphates/deficiency; Phosphates/metabolism; Phospholipases A/genetics; Phospholipases A/metabolism; Phosphorylation; Plant Roots/enzymology; Plant Roots/genetics; Plant Roots/metabolism; Plants, Genetically Modified/enzymology; Plants, Genetically Modified/genetics; Plants, Genetically Modified/metabolism; Sequence Homology, Amino Acid; Signal Transduction/genetics; Signal Transduction/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4JR91

enables

GO:0004620: phospholipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:PLP1

enables

GO:0047372: acylglycerol lipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:PLP1

acts_upstream_of_or_within

GO:0010311: lateral root formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLP1

enables

GO:0004620: phospholipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:PLP5

involved_in

GO:0010311: lateral root formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLP5

enables

GO:0004620: phospholipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:PLP5

enables

GO:0047372: acylglycerol lipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:PLP3

enables

GO:0047372: acylglycerol lipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:PLP3

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLP3

enables

GO:0004620: phospholipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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