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PMID:20045686

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Citation

Lewandowski, JP, Sheehan, KB, Bennett, PE Jr and Boswell, RE (2010) Mago Nashi, Tsunagi/Y14, and Ranshi form a complex that influences oocyte differentiation in Drosophila melanogaster. Dev. Biol. 339:307-19

Abstract

During Drosophila melanogaster oogenesis, a germline stem cell divides forming a cyst of 16 interconnected cells. One cell enters the oogenic pathway, and the remaining 15 differentiate as nurse cells. Although directed transport and localization of oocyte differentiation factors within the single cell are indispensible for selection, maintenance, and differentiation of the oocyte, the mechanisms regulating these events are poorly understood. Mago Nashi and Tsunagi/Y14, core components of the exon junction complex (a multiprotein complex assembled on spliced RNAs), are essential for restricting oocyte fate to a single cell and for localization of oskar mRNA. Here we provide evidence that Mago Nashi and Tsunagi/Y14 form an oogenic complex with Ranshi, a protein with a zinc finger-associated domain and zinc finger domains. Genetic analyses of ranshi reveal that (1) 16-cell cysts are formed, (2) two cells retain synaptonemal complexes, (3) all cells have endoreplicated DNA (as observed in nurse cells), and (4) oocyte-specific cytoplasmic markers accumulate and persist within a single cell but are not localized within the posterior pole of the presumptive oocyte. Our results indicate that Ranshi interacts with the exon junction complex to localize components essential for oocyte differentiation within the posterior pole of the presumptive oocyte.

Links

PubMed PMC2852135 Online version:10.1016/j.ydbio.2009.12.035

Keywords

Animals; Body Patterning; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Differentiation; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/metabolism; Genes, Insect; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Oocytes/cytology; Oocytes/metabolism; Oogenesis/physiology; Phenotype; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:MGN

part_of

GO:0035145: exon-exon junction complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0033378

C

Seeded From UniProt

complete

DROME:RBM8A

part_of

GO:0035145: exon-exon junction complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0002736

C

Seeded From UniProt

complete

DROME:Q9VHM4

involved_in

GO:0030706: germarium-derived oocyte differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VHM4

involved_in

GO:0009994: oocyte differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VHM4

part_of

GO:0035145: exon-exon junction complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0002736

C

Seeded From UniProt

complete

DROME:Q9VHM4

part_of

GO:0035145: exon-exon junction complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0033378

C

Seeded From UniProt

complete


See also

References

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