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PMID:20031913

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Citation

Lin, RC, Park, HJ and Wang, HY (2008) Role of Arabidopsis RAP2.4 in regulating light- and ethylene-mediated developmental processes and drought stress tolerance. Mol Plant 1:42-57

Abstract

Light and the plant hormone ethylene regulate many aspects of plant growth and development in an overlapping and interdependent fashion. Little is known regarding how their signal transduction pathways cross-talk to regulate plant development in a coordinated manner. Here, we report functional characterization of an AP2/DREB-type transcription factor, Arabidopsis RAP2.4, in mediating light and ethylene signaling. Expression of the RAP2.4 gene is down-regulated by light but up-regulated by salt and drought stresses. RAP2.4 protein is constitutively targeted to the nucleus and it can bind to both the ethylene-responsive GCC-box and the dehydration-responsive element (DRE). We show that RAP2.4 protein possesses an intrinsic transcriptional activation activity in yeast cells and that it can activate a reporter gene driven by the DRE cis-element in Arabidopsis protoplasts. Overexpression of RAP2.4 or mutation in RAP2.4 cause altered expression of representative light-, ethylene-, and drought-responsive genes. Although no salient phenotype was observed with a rap2.4 loss-of-function mutant, constitutive overexpression of RAP2.4 results in defects in multiple developmental processes regulated by light and ethylene, including hypocotyl elongation and gravitropism, apical hook formation and cotyledon expansion, flowering time, root elongation, root hair formation, and drought tolerance. Based on these observations, we propose that RAP2.4 acts at or downstream of a converging point of light and ethylene signaling pathways to coordinately regulate multiple developmental processes and stress responses.

Links

PubMed Online version:10.1093/mp/ssm004

Keywords

Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/radiation effects; Arabidopsis Proteins/drug effects; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/radiation effects; Ethylenes/pharmacology; Genes, Plant/drug effects; Genes, Plant/radiation effects; Light; Plant Growth Regulators/pharmacology; RNA, Plant/drug effects; RNA, Plant/genetics; RNA, Plant/radiation effects; RNA, Ribosomal, 18S/drug effects; RNA, Ribosomal, 18S/genetics; RNA, Ribosomal, 18S/radiation effects; Recombinant Fusion Proteins/drug effects; Recombinant Fusion Proteins/metabolism; Recombinant Fusion Proteins/radiation effects; Seedling/drug effects; Seedling/genetics; Seedling/radiation effects; Sodium Chloride/pharmacology; rap GTP-Binding Proteins/drug effects; rap GTP-Binding Proteins/metabolism; rap GTP-Binding Proteins/radiation effects

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:RAP24

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:RAP24

acts_upstream_of_or_within

GO:0009873: ethylene-activated signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RAP24

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RAP24

acts_upstream_of_or_within

GO:0009416: response to light stimulus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RAP24

acts_upstream_of_or_within

GO:0009414: response to water deprivation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RAP24

acts_upstream_of_or_within

GO:0009414: response to water deprivation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RAP24

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:RAP24

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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