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PMID:20010841

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Citation

Weiss, A, Charbonnier, E, Ellertsdóttir, E, Tsirigos, A, Wolf, C, Schuh, R, Pyrowolakis, G and Affolter, M (2010) A conserved activation element in BMP signaling during Drosophila development. Nat. Struct. Mol. Biol. 17:69-76

Abstract

The transforming growth factor beta (TGF-beta) family member Decapentaplegic (Dpp) is a key regulator of patterning and growth in Drosophila development. Previous studies have identified a short DNA motif called the silencer element (SE), which recruits a trimeric Smad complex and the repressor Schnurri to downregulate target enhancers upon Dpp signaling. We have now isolated the minimal enhancer of the dad gene and discovered a short motif we termed the activating element (AE). The AE is similar to the SE and recruits the Smad proteins via a conserved mechanism. However, the AE and SE differ at important nucleotide positions. As a consequence, the AE does not recruit Schnurri but rather integrates repressive input by the default repressor Brinker and activating input by the Smad signal transducers Mothers against Dpp (Mad) and Medea via competitive DNA binding. The AE allows the identification of hitherto unknown direct Dpp targets and is functionally conserved in vertebrates.

Links

PubMed Online version:10.1038/nsmb.1715

Keywords

Animals; Base Sequence; Computational Biology; Drosophila/embryology; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Electrophoretic Mobility Shift Assay; Enhancer Elements, Genetic/genetics; Gene Expression Regulation, Developmental/genetics; Immunohistochemistry; In Situ Hybridization; Molecular Sequence Data; Sequence Analysis, DNA; Signal Transduction/physiology; Silencer Elements, Transcriptional/genetics; Silencer Elements, Transcriptional/physiology; Zebrafish/embryology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:O62609

involved_in

GO:0030509: BMP signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:O62609

enables

GO:0000977: RNA polymerase II transcription regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:O62609

enables

GO:0001228: DNA-binding transcription activator activity, RNA polymerase II-specific

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:O62609

part_of

GO:0005667: transcription regulator complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0011648

C

Seeded From UniProt

complete

DROME:O62609

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:MAD

enables

GO:0000977: RNA polymerase II transcription regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:MAD

enables

GO:0001228: DNA-binding transcription activator activity, RNA polymerase II-specific

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:MAD

part_of

GO:0005667: transcription regulator complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0011655

C

Seeded From UniProt

complete

DROME:Q9XTN4

involved_in

GO:0030514: negative regulation of BMP signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9XTN4

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9XTN4

enables

GO:0000977: RNA polymerase II transcription regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:Q9XTN4

enables

GO:0001227: DNA-binding transcription repressor activity, RNA polymerase II-specific

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:MAD

involved_in

GO:0030509: BMP signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:MAD

part_of

GO:0005667: transcription factor complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0011655

C

Seeded From UniProt

complete

DROME:MAD

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:MAD

enables

GO:0001228: DNA-binding transcription activator activity, RNA polymerase II-specific

ECO:0000314: direct assay evidence used in manual assertion

F

part_of:(GO:0030509)

Seeded From UniProt

complete

DROME:MAD

enables

GO:0000977: RNA polymerase II regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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