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PMID:19969521

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Citation

Li, WF, Perry, PJ, Prafulla, NN and Schmidt, W (2010) Ubiquitin-specific protease 14 (UBP14) is involved in root responses to phosphate deficiency in Arabidopsis. Mol Plant 3:212-23

Abstract

A mutant isolated from a screen of EMS-mutagenized Arabidopsis lines, per1, showed normal root hair development under control conditions but displayed an inhibited root hair elongation phenotype upon Pi deficiency. Additionally, the per1 mutant exhibited a pleiotropic phenotype under control conditions, resembling Pi-deficient plants in several aspects. Inhibition of root hair elongation upon growth on low Pi media was reverted by treatment with the Pi analog phosphite, suggesting that the mutant phenotype is not caused by a lack of Pi. Reciprocal grafting experiments revealed that the mutant rootstock is sufficient to cause the phenotype. Complementation analyses showed that the PER1 gene encodes an ubiquitin-specific protease, UBP14. The mutation caused a synonymous substitution in the 12th exon of this gene, resulting in a lower abundance of the UBP14 protein, probably as a consequence of reduced translation efficiency. Transcriptional profiling of per1 and wild-type plants subjected to short-term Pi starvation revealed genes that may be important for the signaling of Pi deficiency. We conclude that UBP14 function is crucial for adapting root development to the prevailing local availability of phosphate.

Links

PubMed Online version:10.1093/mp/ssp086

Keywords

Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Endopeptidases/genetics; Endopeptidases/metabolism; Gene Expression Regulation, Plant/genetics; Gene Expression Regulation, Plant/physiology; Microscopy; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Phosphates/deficiency; Phosphates/metabolism; Plant Roots/enzymology; Plant Roots/genetics; Plant Roots/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:UBP14

enables

GO:0004843: thiol-dependent deubiquitinase

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:UBP14

acts_upstream_of_or_within

GO:0048767: root hair elongation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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