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PMID:19966281

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Citation

Koike, C, Obara, T, Uriu, Y, Numata, T, Sanuki, R, Miyata, K, Koyasu, T, Ueno, S, Funabiki, K, Tani, A, Ueda, H, Kondo, M, Mori, Y, Tachibana, M and Furukawa, T (2010) TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade. Proc. Natl. Acad. Sci. U.S.A. 107:332-7

Abstract

An essential step in intricate visual processing is the segregation of visual signals into ON and OFF pathways by retinal bipolar cells (BCs). Glutamate released from photoreceptors modulates the photoresponse of ON BCs via metabotropic glutamate receptor 6 (mGluR6) and G protein (Go) that regulates a cation channel. However, the cation channel has not yet been unequivocally identified. Here, we report a mouse TRPM1 long form (TRPM1-L) as the cation channel. We found that TRPM1-L localization is developmentally restricted to the dendritic tips of ON BCs in colocalization with mGluR6. TRPM1 null mutant mice completely lose the photoresponse of ON BCs but not that of OFF BCs. In the TRPM1-L-expressing cells, TRPM1-L functions as a constitutively active nonselective cation channel and its activity is negatively regulated by Go in the mGluR6 cascade. These results demonstrate that TRPM1-L is a component of the ON BC transduction channel downstream of mGluR6 in ON BCs.

Links

PubMed PMC2806705 Online version:10.1073/pnas.0912730107

Keywords

Animals; CHO Cells; Cricetinae; Cricetulus; Electrophysiology; Humans; Light; Light Signal Transduction/physiology; Mice; Mice, Knockout; Molecular Sequence Data; Open Reading Frames; Protein Isoforms/genetics; Protein Isoforms/metabolism; Receptors, Metabotropic Glutamate/genetics; Receptors, Metabotropic Glutamate/metabolism; Retinal Bipolar Cells/cytology; Retinal Bipolar Cells/physiology; TRPM Cation Channels/genetics; TRPM Cation Channels/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:BSN

located_in

GO:0045202: synapse

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:GNAS2

located_in

GO:0030425: dendrite

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:TRPM1

enables

GO:0005261: cation channel activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:TRPM1

acts_upstream_of_or_within

GO:0007165: signal transduction

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1351343

P

Seeded From UniProt

complete

MOUSE:TRPM1

located_in

GO:0035841: new growing cell tip

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000749)

Seeded From UniProt

complete

MOUSE:TRPM1

acts_upstream_of_or_within

GO:0046548: retinal rod cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:4421441

P

Seeded From UniProt

complete

MOUSE:TRPM1

acts_upstream_of_or_within

GO:0071482: cellular response to light stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:4421441

P

  • occurs_in:(CL:0000749)

Seeded From UniProt

complete

MOUSE:GRM6

acts_upstream_of_or_within

GO:0007165: signal transduction

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1330305

P

Seeded From UniProt

complete

MOUSE:GRM6

located_in

GO:0035841: new growing cell tip

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:ALEX

located_in

GO:0030425: dendrite

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:GNAS1

located_in

GO:0030425: dendrite

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:GNAS3

located_in

GO:0030425: dendrite

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:BSN

part_of

GO:0045202: synapse

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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