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PMID:19965874

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Citation

Ahn, YO, Shimizu, B, Sakata, K, Gantulga, D, Zhou, C, Zhou, Z, Bevan, DR and Esen, A (2010) Scopolin-hydrolyzing beta-glucosidases in roots of Arabidopsis. Plant Cell Physiol. 51:132-43

Abstract

Three beta-glucosidases (At1g66270-BGLU21, At1g66280-BGLU22, and At3g09260-BGLU23) were purified from the roots of Arabidopsis and their cDNAs were expressed in insect cells. In addition, two beta-glucosidase binding protein cDNAs (At3g16420; PBPI and At3g16430; PBPII) were expressed in Escherichia coli and their protein products purified. These binding proteins interact with beta-glucosidases and activate them. BGLU21, 22 and 23 hydrolyzed the natural substrate scopolin specifically and also hydrolyzed to some extent substrates whose aglycone moiety is similar to scopolin (e.g. esculin and 4-MU-glucoside). In contrast, they hydrolyzed poorly DIMBOA-glucoside and did not hydrolyze pNP- and oNP-glucosides. We determined the physicochemical properties of native and recombinant BGLUs, and found no differences between them. They were stable in a narrow pH range (5-7.5) and had temperature and pH optima for activity at 35 degrees C and pH 5.5, respectively. As for thermostability, >95% of their activity was retained at 40 degrees C but dramatically decreased at >50 degrees C. The apparent K(m) of native and recombinant enzymes for scopolin was 0.73 and 0.81 mM, respectively, and it was 5.8 and 9.7 mM, respectively, for esculin. Western blot analysis showed that all three enzymes were exclusively expressed in roots of seedlings but not in any other plant part or organ under normal conditions. Furthermore, spatial expression patterns of all eight genes belonging to subfamily 3 were investigated at the transcription level by RT-PCR.

Links

PubMed Online version:10.1093/pcp/pcp174

Keywords

Amino Acid Sequence; Arabidopsis/cytology; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/isolation & purification; Arabidopsis Proteins/metabolism; Carrier Proteins/genetics; Carrier Proteins/isolation & purification; Carrier Proteins/metabolism; Cellulases/genetics; Cellulases/isolation & purification; Cellulases/metabolism; Coumarins/metabolism; Enzyme Activation/physiology; Esculin/metabolism; Gene Expression Regulation, Enzymologic/genetics; Gene Expression Regulation, Plant/genetics; Glucosides/metabolism; Hydrogen-Ion Concentration; Hydrolysis; Hymecromone/analogs & derivatives; Hymecromone/metabolism; Molecular Sequence Data; Plant Roots/cytology; Plant Roots/enzymology; Plant Roots/genetics; Protein Binding/physiology; Protein Stability; Temperature

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:JAL30

enables

GO:0030234: enzyme regulator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:JAL30

acts_upstream_of_or_within

GO:0051336: regulation of hydrolase activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL21

acts_upstream_of_or_within

GO:0006970: response to osmotic stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL21

acts_upstream_of_or_within

GO:0009804: coumarin metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL21

enables

GO:0008422: beta-glucosidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:BGL21

acts_upstream_of_or_within

GO:0071472: cellular response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL21

acts_upstream_of_or_within

GO:0070417: cellular response to cold

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL22

enables

GO:0008422: beta-glucosidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:BGL22

acts_upstream_of_or_within

GO:0071472: cellular response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL22

acts_upstream_of_or_within

GO:0070417: cellular response to cold

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL23

acts_upstream_of_or_within

GO:0006970: response to osmotic stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BGL23

enables

GO:0008422: beta-glucosidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:BGL23

acts_upstream_of_or_within

GO:0070417: cellular response to cold

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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