PMID:19960362

Citation	Köhler, D, Schmidt-Gattung, S and Binder, S (2010) The DEAD-box protein PMH2 is required for efficient group II intron splicing in mitochondria of Arabidopsis thaliana. Plant Mol. Biol. 72:459-67
Abstract	In Arabidopsis thaliana the putative mitochondrial RNA helicases PMH1 and PMH2 are members of the large DEAD-box protein family. Our previous characterization of these proteins revealed that PMH1 and/or PMH2 are part of high molecular weight complexes. Now T-DNA insertion lines were established and characterized for each of these genes. Immunodetection analysis of cell suspension cultures established from pmh1-1 and pmh2-1 mutants revealed that indeed both DEAD-box proteins are detectable in large protein complexes with PMH2 being much more abundant than PMH1. In plants the knockout of PMH2 leads to reduced group II intron splicing efficiency. In addition the steady-state levels of several mature mitochondrial mRNAs are decreased while transcription is not influenced. This molecular phenotype suggests that PMH2 acts at the posttranscriptional level with a potential function as RNA chaperone required for formation or maintenance of complex RNA secondary structures of introns rather than a direct role in splicing. In contrast, the investigation of a pmh1-1 knockout line did not reveal any influence of this protein on processing and abundance of mitochondrial transcripts.
Links	PubMed Online version:10.1007/s11103-009-9584-9
Keywords	Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; DEAD-box RNA Helicases/chemistry; DEAD-box RNA Helicases/genetics; DEAD-box RNA Helicases/metabolism; Genes, Plant; Introns; Macromolecular Substances; Mitochondria/metabolism; Molecular Chaperones/chemistry; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Mutagenesis, Insertional; Plants, Genetically Modified; RNA Splicing; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Plant/genetics; RNA, Plant/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:RH53	part_of	GO:0032991: protein-containing complex	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
ARATH:RH53	acts_upstream_of_or_within	GO:0009409: response to cold	ECO:0000270: expression pattern evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:RH53	acts_upstream_of_or_within	GO:0000373: Group II intron splicing	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:RH9	part_of	GO:0032991: protein-containing complex	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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