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PMID:19948887

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Citation

Quan, TK and Hartzog, GA (2010) Histone H3K4 and K36 methylation, Chd1 and Rpd3S oppose the functions of Saccharomyces cerevisiae Spt4-Spt5 in transcription. Genetics 184:321-34

Abstract

Spt4-Spt5, a general transcription elongation factor for RNA polymerase II, also has roles in chromatin regulation. However, the relationships between these functions are not clear. Previously, we isolated suppressors of a Saccharomyces cerevisiae spt5 mutation in genes encoding members of the Paf1 complex, which regulates several cotranscriptional histone modifications, and Chd1, a chromatin remodeling enzyme. Here, we show that this suppression of spt5 can result from loss of histone H3 lysines 4 or 36 methylation, or reduced recruitment of Chd1 or the Rpd3S complex. These spt5 suppressors also rescue the synthetic growth defects observed in spt5 mutants that also lack elongation factor TFIIS. Using a FLO8 reporter gene, we found that a chd1 mutation caused cryptic initiation of transcription. We further observed enhancement of cryptic initiation in chd1 isw1 mutants and increased histone acetylation in a chd1 mutant. We suggest that, as previously proposed for H3 lysine 36 methylation and the Rpd3S complex, H3 lysine 4 methylation and Chd1 function to maintain normal chromatin structures over transcribed genes, and that one function of Spt4-Spt5 is to help RNA polymerase II overcome the repressive effects of these histone modifications and chromatin regulators on transcription.

Links

PubMed PMC2828714 Online version:10.1534/genetics.109.111526

Keywords

Acetylation; Chromatin/metabolism; Chromosomal Proteins, Non-Histone/genetics; Chromosomal Proteins, Non-Histone/metabolism; Conserved Sequence; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/metabolism; Histone Deacetylases/metabolism; Histones/chemistry; Histones/metabolism; Kinetics; Lysine/metabolism; Methylation; Mutation; Nuclear Proteins/metabolism; Nucleosomes/metabolism; Protein Structure, Tertiary; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription, Genetic; Transcriptional Elongation Factors/genetics; Transcriptional Elongation Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:CHD1

involved_in

GO:0001178: regulation of transcriptional start site selection at RNA polymerase II promoter

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000449

P

Seeded From UniProt

complete

YEAST:CHD1

involved_in

GO:2000616: negative regulation of histone H3-K9 acetylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:CHD1

involved_in

GO:0071441: negative regulation of histone H3-K14 acetylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:ISW1

involved_in

GO:0001178: regulation of transcriptional start site selection at RNA polymerase II promoter

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000966

P

Seeded From UniProt

complete

YEAST:SET2

involved_in

GO:2000616: negative regulation of histone H3-K9 acetylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SET2

involved_in

GO:0071441: negative regulation of histone H3-K14 acetylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:RCO1

involved_in

GO:0006368: transcription elongation from RNA polymerase II promoter

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004470

P

Seeded From UniProt

complete

YEAST:EAF3

involved_in

GO:0006368: transcription elongation from RNA polymerase II promoter

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004470

P

Seeded From UniProt

complete

YEAST:RPD3

involved_in

GO:0006368: transcription elongation from RNA polymerase II promoter

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004470

P

Seeded From UniProt

complete


See also

References

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