GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19945479

From GONUTS
Jump to: navigation, search
Citation

Mao, W, Hunt, HD and Cheng, HH (2010) Cloning and functional characterization of chicken stem cell antigen 2. Dev. Comp. Immunol. 34:360-8

Abstract

Stem cell antigen 2 (SCA2) is a Ly6 family member whose function is largely unknown. To characterize biological properties and tissue distribution of chicken SCA2, SCA2 was expressed in E. coli, purified, and a polyclonal antibody developed. Utilizing the polyclonal antibody, SCA2 is a 13 kDa cell surface protein anchored by a glycosyl-phosphatidylinositol (GPI) moiety. SCA2 is expressed in connective tissues of thymus and bursa based on immunohistochemistry, immunoprecipitation, and western blots. In bursal follicles, SCA2 is specifically expressed on the cortical-medullary epithelial cells (CMEC) surrounded by MHC class II presenting cells. Expression profiles of bursal cells induced by contact with SCA2-expressing cells shows down-regulation of numerous genes including CD79B, B cell linker (BLNK), spleen tyrosine kinase (SYK), and gamma 2-phospholipase C (PLCG2) that are involved in the B cell receptor (BCR) and immune response signaling pathways. These results suggest chicken SCA2 plays a role in regulating B lymphocytes.

Links

PubMed Online version:10.1016/j.dci.2009.11.008

Keywords

Amino Acid Sequence; Animals; B-Lymphocytes/immunology; B-Lymphocytes/metabolism; Blotting, Western; Cell Separation; Chickens/genetics; Chickens/immunology; Chickens/metabolism; Cloning, Molecular; Flow Cytometry; Gene Expression Regulation; Immunohistochemistry; Immunoprecipitation; Membrane Proteins/genetics; Membrane Proteins/immunology; Membrane Proteins/metabolism; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction/immunology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:A0A1D5P7D9

located_in

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0002107)
  • part_of:(CL:0000182)
  • part_of:(CL:0000115)|part_of:(UBERON:0001250)|part_of:(UBERON:0000991)|part_of:(UBERON:0000948)|part_of:(UBERON:0002048)|part_of:(CL:0002293)|part_of:(UBERON:0003905)

Seeded From UniProt

complete

CHICK:LY6E

located_in

GO:0031225: anchored component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:LY6E

located_in

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0002107)
  • part_of:(CL:0000182)
  • part_of:(CL:0000115)|part_of:(UBERON:0001250)|part_of:(UBERON:0000991)|part_of:(UBERON:0000948)|part_of:(UBERON:0002048)|part_of:(CL:0002293)|part_of:(UBERON:0003905)

Seeded From UniProt

complete

CHICK:LY6E

located_in

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:LY6E

involved_in

GO:0002320: lymphoid progenitor cell differentiation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:LY6E

involved_in

GO:0008283: cell population proliferation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

CHICK:LY6E

part_of

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0002107)
  • part_of:(CL:0000182)
  • part_of:(CL:0000115)|part_of:(UBERON:0001250)|part_of:(UBERON:0000991)|part_of:(UBERON:0000948)|part_of:(UBERON:0002048)|part_of:(CL:0002293)|part_of:(UBERON:0003905)

Seeded From UniProt

complete

CHICK:LY6E

part_of

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:LY6E

part_of

GO:0031225: anchored component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.