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PMID:19934015

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Citation

Bertet, C, Rauzi, M and Lecuit, T (2009) Repression of Wasp by JAK/STAT signalling inhibits medial actomyosin network assembly and apical cell constriction in intercalating epithelial cells. Development 136:4199-212

Abstract

Tissue morphogenesis requires stereotyped cell shape changes, such as apical cell constriction in the mesoderm and cell intercalation in the ventrolateral ectoderm of Drosophila. Both processes require force generation by an actomyosin network. The subcellular localization of Myosin-II (Myo-II) dictates these different morphogenetic processes. In the intercalating ectoderm Myo-II is mostly cortical, but in the mesoderm Myo-II is concentrated in a medial meshwork. We report that apical constriction is repressed by JAK/STAT signalling in the lateral ectoderm independently of Twist. Inactivation of the JAK/STAT pathway causes germband extension defects because of apical constriction ventrolaterally. This is associated with ectopic recruitment of Myo-II in a medial web, which causes apical cell constriction as shown by laser nanosurgery. Reducing Myo-II levels rescues the JAK/STAT mutant phenotype, whereas overexpression of the Myo-II heavy chain (also known as Zipper), or constitutive activation of its regulatory light chain, does not cause medial accumulation of Myo-II nor apical constriction. Thus, JAK/STAT controls Myo-II localization by additional mechanisms. We show that regulation of actin polymerization by Wasp, but not by Dia, is important in this process. Constitutive activation of Wasp, a branched actin regulator, causes apical cell constriction and promotes medial 'web' formation. Wasp is inactivated at the cell cortex in the germband by JAK/STAT signalling. Lastly, wasp mutants rescue the normal cortical enrichment of Myo-II and inhibit apical constriction in JAK/STAT mutants, indicating that Wasp is an effector of JAK/STAT signalling in the germband. We discuss possible models for the role of Wasp activity in the regulation of Myo-II distribution.

Links

PubMed Online version:10.1242/dev.040402

Keywords

Actomyosin/metabolism; Animals; Cell Polarity/physiology; Cytoskeleton/metabolism; Drosophila/embryology; Drosophila/physiology; Drosophila Proteins/antagonists & inhibitors; Drosophila Proteins/physiology; Ectoderm/embryology; Ectoderm/physiology; Epithelial Cells/cytology; Epithelial Cells/physiology; Janus Kinases/physiology; Mesoderm/embryology; Mesoderm/physiology; Morphogenesis/physiology; Myosin Type II/physiology; STAT Transcription Factors/physiology; Signal Transduction; Wiskott-Aldrich Syndrome Protein/antagonists & inhibitors; Wiskott-Aldrich Syndrome Protein/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:O96525

involved_in

GO:0016476: regulation of embryonic cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:O96525

involved_in

GO:0003383: apical constriction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:O96525

involved_in

GO:0060031: mediolateral intercalation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:DIA

involved_in

GO:0016476: regulation of embryonic cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:DIA

involved_in

GO:0003383: apical constriction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:JAK

involved_in

GO:0016476: regulation of embryonic cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:JAK

involved_in

GO:0060031: mediolateral intercalation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q86B74

involved_in

GO:0016476: regulation of embryonic cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q86B74

involved_in

GO:0030833: regulation of actin filament polymerization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q86B74

involved_in

GO:0003383: apical constriction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MZC7

involved_in

GO:0016476: regulation of embryonic cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MZC7

involved_in

GO:0003383: apical constriction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MZC7

involved_in

GO:0060031: mediolateral intercalation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VAT0

involved_in

GO:0016476: regulation of embryonic cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VAT0

involved_in

GO:0030833: regulation of actin filament polymerization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VAT0

involved_in

GO:0003383: apical constriction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VWW4

involved_in

GO:0016476: regulation of embryonic cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VWW4

involved_in

GO:0003383: apical constriction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VWW4

involved_in

GO:0060031: mediolateral intercalation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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