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PMID:19913509

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Citation

Uemura, M, Hara, K, Shitara, H, Ishii, R, Tsunekawa, N, Miura, Y, Kurohmaru, M, Taya, C, Yonekawa, H, Kanai-Azuma, M and Kanai, Y (2010) Expression and function of mouse Sox17 gene in the specification of gallbladder/bile-duct progenitors during early foregut morphogenesis. Biochem. Biophys. Res. Commun. 391:357-63

Abstract

In early-organogenesis-stage mouse embryos, the posteroventral foregut endoderm adjacent to the heart tube gives rise to liver, ventral pancreas and gallbladder. Hepatic and pancreatic primordia become specified in the posterior segment of the ventral foregut endoderm at early somite stages. The mechanisms for demarcating gallbladder and bile duct primordium, however, are poorly understood. Here, we demonstrate that the gallbladder and bile duct progenitors are specified in the paired lateral endoderm domains outside the heart field at almost the same timing as hepatic and pancreatic induction. In the anterior definitive endoderm, Sox17 reactivation occurs in a certain population within the most lateral domains posterolateral to the anterior intestinal portal (AIP) lip on both the left and right sides. During foregut formation, the paired Sox17-positive domains expand ventromedially to merge in the midline of the AIP lip and become localized between the liver and pancreatic primordia. In Sox17-null embryos, these lateral domains are missing, resulting in a complete loss of the gallbladder/bile-duct structure. Chimera analyses revealed that Sox17-null endoderm cells in the posteroventral foregut do not display any gallbladder/bile-duct molecular characters. Our findings show that Sox17 functions cell-autonomously to specify gallbladder/bile-duct in the mouse embryo.

Links

PubMed Online version:10.1016/j.bbrc.2009.11.063

Keywords

Animals; Bile Ducts/abnormalities; Bile Ducts/embryology; Bile Ducts/metabolism; Body Patterning; Embryo, Mammalian/metabolism; Endoderm/metabolism; Female; Gallbladder/abnormalities; Gallbladder/embryology; Gallbladder/metabolism; Gene Expression Regulation, Developmental; HMGB Proteins/genetics; HMGB Proteins/physiology; Intestines/embryology; Intestines/metabolism; Mice; Mice, Inbred C57BL; Morphogenesis; SOXF Transcription Factors/genetics; SOXF Transcription Factors/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:SOX17

acts_upstream_of_or_within

GO:0021903: rostrocaudal neural tube patterning

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2179440

P

Seeded From UniProt

complete

MOUSE:SOX17

acts_upstream_of_or_within

GO:0007492: endoderm development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2179440

P

Seeded From UniProt

complete

MOUSE:SOX17

acts_upstream_of_or_within

GO:0061010: gall bladder development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2179440

P

Seeded From UniProt

complete

MOUSE:SOX17

acts_upstream_of_or_within

GO:0061009: common bile duct development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SOX17

acts_upstream_of_or_within

GO:0048568: embryonic organ development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:2179440

P

Seeded From UniProt

complete


See also

References

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