GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19896942

From GONUTS
Jump to: navigation, search
Citation

Guranowski, A, Wojdyła, AM, Zimny, J, Wypijewska, A, Kowalska, J, Jemielity, J, Davis, RE and Bieganowski, P (2010) Dual activity of certain HIT-proteins: A. thaliana Hint4 and C. elegans DcpS act on adenosine 5'-phosphosulfate as hydrolases (forming AMP) and as phosphorylases (forming ADP). FEBS Lett. 584:93-8

Abstract

Histidine triad (HIT)-family proteins interact with different mono- and dinucleotides and catalyze their hydrolysis. During a study of the substrate specificity of seven HIT-family proteins, we have shown that each can act as a sulfohydrolase, catalyzing the liberation of AMP from adenosine 5'-phosphosulfate (APS or SO(4)-pA). However, in the presence of orthophosphate, Arabidopsis thaliana Hint4 and Caenorhabditis elegans DcpS also behaved as APS phosphorylases, forming ADP. Low pH promoted the phosphorolytic and high pH the hydrolytic activities. These proteins, and in particular Hint4, also catalyzed hydrolysis or phosphorolysis of some other adenylyl-derivatives but at lower rates than those for APS cleavage. A mechanism for these activities is proposed and the possible role of some HIT-proteins in APS metabolism is discussed.

Links

PubMed Online version:10.1016/j.febslet.2009.11.003

Keywords

Adenosine Diphosphate/biosynthesis; Adenosine Monophosphate/biosynthesis; Adenosine Phosphosulfate/metabolism; Animals; Arabidopsis/enzymology; Caenorhabditis elegans/enzymology; Caenorhabditis elegans Proteins/metabolism; Hydrogen-Ion Concentration; Hydrolases/metabolism; Hydrolysis; Multienzyme Complexes/metabolism; Phosphoric Monoester Hydrolases/metabolism; Phosphorylation; Pyrophosphatases/metabolism; Substrate Specificity; Sulfatases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:D8FRQ0

involved_in

GO:0009150: purine ribonucleotide metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:D8FRQ0

involved_in

GO:0006790: sulfur compound metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:D8FRQ0

enables

GO:0004780: sulfate adenylyltransferase (ADP) activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:D8FRQ0

enables

GO:0047627: adenylylsulfatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:HINT3

acts_upstream_of_or_within

GO:0006790: sulfur compound metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:HINT3

acts_upstream_of_or_within

GO:0009150: purine ribonucleotide metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:HINT3

enables

GO:0047627: adenylylsulfatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:DCPS

involved_in

GO:0009150: purine ribonucleotide metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:DCPS

involved_in

GO:0006790: sulfur compound metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:DCPS

enables

GO:0004780: sulfate adenylyltransferase (ADP) activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:DCPS

enables

GO:0047627: adenylylsulfatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:HINT4

acts_upstream_of_or_within

GO:0006790: sulfur compound metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:HINT4

acts_upstream_of_or_within

GO:0009150: purine ribonucleotide metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:HINT4

enables

GO:0004780: sulfate adenylyltransferase (ADP) activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:HINT4

enables

GO:0047627: adenylylsulfatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:HINT1

acts_upstream_of_or_within

GO:0006790: sulfur compound metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:HINT1

acts_upstream_of_or_within

GO:0009150: purine ribonucleotide metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:HINT1

enables

GO:0047627: adenylylsulfatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:BH140

acts_upstream_of_or_within

GO:0006790: sulfur compound metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BH140

acts_upstream_of_or_within

GO:0009150: purine ribonucleotide metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BH140

enables

GO:0047627: adenylylsulfatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.