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PMID:19880396

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Citation

Fukao, Y, Ferjani, A, Fujiwara, M, Nishimori, Y and Ohtsu, I (2009) Identification of zinc-responsive proteins in the roots of Arabidopsis thaliana using a highly improved method of two-dimensional electrophoresis. Plant Cell Physiol. 50:2234-9

Abstract

Zinc (Zn) is an essential micronutrient for various physiological and metabolic processes in plants, although it is toxic in excess. To understand better Zn-responsive proteins, we developed a highly improved method of isoelectric focusing (IEF) in which whole lysate from Arabidopsis roots is subjected to IEF without any desalting steps. In this method, samples extracted with lysis buffer containing 1.5% SDS can also be directly applied to IEF. By applying this method to Zn-treated Arabidopsis roots, 10 up-regulated and 17 down-regulated proteins were identified, 15 of which showed a significant correlation with previously reported transcriptomic data.

Links

PubMed Online version:10.1093/pcp/pcp154

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Culture Media; Electrophoresis, Gel, Two-Dimensional/methods; Gene Expression Regulation, Plant; Plant Roots/genetics; Plant Roots/metabolism; Zinc/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4JRY2

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4JTK8

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:JAL33

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PSA2A

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:METE1

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GSTF9

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:O80889

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PSA6A

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODM1

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PER22

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GSTF2

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TPIS

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MDHC1

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RBG7

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RBG8

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:FRI1

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RLA02

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MDAR2

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:EF1G2

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DHAR1

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TCPZA

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Q9S7W4

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ICDHC

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PDI11

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ML328

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PDI11

involved_in

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RBG7

involved_in

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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