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PMID:19878652

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Citation

Peng, L, Ye, L, Dong, G, Ren, LB, Wang, CL, Xu, P and Zhou, XD (2009) WNT5A inhibits human dental papilla cell proliferation and migration. Biochem. Biophys. Res. Commun. 390:1072-8

Abstract

WNT proteins are a large family of cysteine-rich secreted molecules that are linked to both canonical and non-canonical signal pathways, and have been implicated in oncogenesis and tissue development. Canonical WNT proteins have been proven to play critical roles in tooth development, while little is known about the role of non-canonical WNT proteins such as WNT5A. In this study, WNT5A was localized to human dental papilla tissue and human dental papilla cells (HDPCs) cultured in vitro, using immunochemistry and RT-PCR. Recombinant adenovirus encoding full-length Wnt5a cDNA was constructed to investigate the biological role of WNT5A on HDPCs. The BrdU incorporation assay, the MTT assay and flow cytometric analysis showed that over-expression of Wnt5a strongly inhibited the proliferation of HDPCs in vitro. Wound healing and transwell migration assays indicated that over-expression of WNT5A reduced migration of HDPCs. In conclusion, our results showed that WNT5A negatively regulates both proliferation and migration of HDPCs, suggesting its important role in odontogenesis via controlling the HDPCs.

Links

PubMed Online version:10.1016/j.bbrc.2009.10.136

Keywords

Adenoviridae; Cadaver; Cell Movement; Cell Proliferation; Cells, Cultured; Dental Papilla/cytology; Dental Papilla/metabolism; Dental Papilla/physiology; Fetus; Humans; Odontogenesis; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/physiology; Wnt Proteins/genetics; Wnt Proteins/physiology; Wound Healing

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:WNT5A

involved_in

GO:0042060: wound healing

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:WNT5A

involved_in

GO:0072201: negative regulation of mesenchymal cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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