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PMID:19854947
| Citation |
Graham, JE, Sivanathan, V, Sherratt, DJ and Arciszewska, LK (2010) FtsK translocation on DNA stops at XerCD-dif. Nucleic Acids Res. 38:72-81 |
|---|---|
| Abstract |
Escherichia coli FtsK is a powerful, fast, double-stranded DNA translocase, which can strip proteins from DNA. FtsK acts in the late stages of chromosome segregation by facilitating sister chromosome unlinking at the division septum. KOPS-guided DNA translocation directs FtsK towards dif, located within the replication terminus region, ter, where FtsK activates XerCD site-specific recombination. Here we show that FtsK translocation stops specifically at XerCD-dif, thereby preventing removal of XerCD from dif and allowing activation of chromosome unlinking by recombination. Stoppage of translocation at XerCD-dif is accompanied by a reduction in FtsK ATPase and is not associated with FtsK dissociation from DNA. Specific stoppage at recombinase-DNA complexes does not require the FtsKgamma regulatory subdomain, which interacts with XerD, and is not dependent on either recombinase-mediated DNA cleavage activity, or the formation of synaptic complexes. |
| Links |
PubMed PMC2800217 Online version:10.1093/nar/gkp843 |
| Keywords |
Adenosine Triphosphatases/metabolism; Binding Sites; DNA/metabolism; DNA Cleavage; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Integrases/metabolism; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Protein Structure, Tertiary; Protein Transport; Recombination, Genetic |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0016887: ATPase |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007059: chromosome segregation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007059: chromosome segregation |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P0A8P6 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0015616: DNA translocase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016887: ATPase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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