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PMID:19846542

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Citation

Whitley, P, Hinz, S and Doughty, J (2009) Arabidopsis FAB1/PIKfyve proteins are essential for development of viable pollen. Plant Physiol. 151:1812-22

Abstract

Phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P(2)] is a phospholipid that has a role in controlling membrane trafficking events in yeast and animal cells. The function of this lipid in plants is unknown, although its synthesis has been shown to be up-regulated upon osmotic stress in plant cells. PtdIns(3,5)P(2) is synthesized by the PIKfyve/Fab1 family of proteins, with two orthologs, FAB1A and FAB1B, being present in Arabidopsis (Arabidopsis thaliana). In this study, we attempt to address the role of this lipid by analyzing the phenotypes of plants mutated in FAB1A and FAB1B. It was not possible to generate plants homozygous for mutations in both genes, although single mutants were isolated. Both homozygous single mutant plant lines exhibited a leaf curl phenotype that was more marked in FAB1B mutants. Genetic transmission analysis revealed that failure to generate double mutant lines was entirely due to inviability of pollen carrying mutant alleles of both FAB1A and FAB1B. This pollen displayed severe defects in vacuolar reorganization following the first mitotic division of development. The presence of abnormally large vacuoles in pollen at the tricellular stage resulted in the collapse of the majority of grains carrying both mutant alleles. This demonstrates a crucial role for PtdIns(3,5)P(2) in modulating the dynamics of vacuolar rearrangement essential for successful pollen development. Taken together, our results are consistent with PtdIns(3,5)P(2) production being central to cellular responses to changes in osmotic conditions.

Links

PubMed PMC2785992 Online version:10.1104/pp.109.146159

Keywords

Arabidopsis/cytology; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/ultrastructure; Arabidopsis Proteins/metabolism; Gene Knockout Techniques; Mutation/genetics; Neutral Red/metabolism; Phenotype; Phosphotransferases (Alcohol Group Acceptor)/metabolism; Phylogeny; Plant Leaves/enzymology; Pollen/cytology; Pollen/enzymology; Pollen/growth & development; Pollen/ultrastructure; Reproduction; Seeds/enzymology; Seeds/growth & development; Staining and Labeling; Tissue Survival; Vacuoles/enzymology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:FAB1A

acts_upstream_of_or_within

GO:0007033: vacuole organization

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT4G33240

P

Seeded From UniProt

complete

ARATH:FAB1A

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT4G33240

P

Seeded From UniProt

complete

ARATH:FAB1A

enables

GO:0000285: 1-phosphatidylinositol-3-phosphate 5-kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

SGD:S000001915

F

Seeded From UniProt

complete

ARATH:FAB1B

acts_upstream_of_or_within

GO:0007033: vacuole organization

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT4G33240

P

Seeded From UniProt

complete

ARATH:FAB1B

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT4G33240

P

Seeded From UniProt

complete

ARATH:FAB1B

enables

GO:0000285: 1-phosphatidylinositol-3-phosphate 5-kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

SGD:S000001915

F

Seeded From UniProt

complete


See also

References

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