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PMID:19846433

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Citation

Du, J, Chen, T, Zou, X, Xiong, B and Lu, G (2010) Dppa2 knockdown-induced differentiation and repressed proliferation of mouse embryonic stem cells. J. Biochem. 147:265-71

Abstract

Developmental pluripotency-associated 2 (Dppa2) gene is one of the genes recently identified to be expressed specifically in pluripotent cells. To investigate the role of Dppa2 in mouse embryonic stem (ES) cells, we examined its expression during differentiation and performed knockdown of Dppa2 in mouse ES cells. Our results showed that the expression of Dppa2 decreased markedly in differentiated cells. Dppa2 knockdown induced the differentiation of mouse ES cells, as indicated by reduced alkaline phosphatase activity, slightly downregulated expression of the putative pluripotency marker genes Oct4 and Nanog and increased expression of early differentiation marker genes, such as Fst and Psx1. Moreover, reduced expression of Dppa2 also repressed cell proliferation activity as shown by the 5-bromo-2-deoxyuridine incorporation assay. Hence, Dppa2 might play a role in maintenance of the undifferentiated state and proliferation of ES cells.

Links

PubMed Online version:10.1093/jb/mvp161

Keywords

Animals; Cell Differentiation/genetics; Cell Differentiation/physiology; Cell Line; Cell Proliferation; Embryonic Stem Cells/cytology; Embryonic Stem Cells/metabolism; Fluorescent Antibody Technique; Immunohistochemistry; Mice; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; RNA Interference; Reverse Transcriptase Polymerase Chain Reaction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:DPPA2

involved_in

GO:0019827: stem cell population maintenance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DPPA2

involved_in

GO:2000648: positive regulation of stem cell proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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