GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19826475

From GONUTS
Jump to: navigation, search
Citation

Schouest, KR, Kurasawa, Y, Furuta, T, Hisamoto, N, Matsumoto, K and Schumacher, JM (2009) The germinal center kinase GCK-1 is a negative regulator of MAP kinase activation and apoptosis in the C. elegans germline. PLoS ONE 4:e7450

Abstract

The germinal center kinases (GCK) constitute a large, highly conserved family of proteins that has been implicated in a wide variety of cellular processes including cell growth and proliferation, polarity, migration, and stress responses. Although diverse, these functions have been attributed to an evolutionarily conserved role for GCKs in the activation of ERK, JNK, and p38 MAP kinase pathways. In addition, multiple GCKs from different species promote apoptotic cell death. In contrast to these paradigms, we found that a C. elegans GCK, GCK-1, functions to inhibit MAP kinase activation and apoptosis in the C. elegans germline. In the absence of GCK-1, a specific MAP kinase isoform is ectopically activated and oocytes undergo abnormal development. Moreover, GCK-1- deficient animals display a significant increase in germ cell death. Our results suggest that individual germinal center kinases act in mechanistically distinct ways and that these functions are likely to depend on organ- and developmental-specific contexts.

Links

PubMed PMC2757678 Online version:10.1371/journal.pone.0007450

Keywords

Animals; Apoptosis; Caenorhabditis elegans; Caenorhabditis elegans Proteins/metabolism; Cell Proliferation; Cytoplasm/metabolism; Enzyme Activation; Female; Gene Expression Regulation, Enzymologic; Male; Mitogen-Activated Protein Kinase 1/metabolism; Mitogen-Activated Protein Kinases/metabolism; Models, Biological; Oocytes/metabolism; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases/metabolism; RNA Interference

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:GCK1

involved_in

GO:0060281: regulation of oocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00088246

P

Seeded From UniProt

complete

CAEEL:GCK1

enables

GO:0051019: mitogen-activated protein kinase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P39745-2

F

Seeded From UniProt

complete

CAEEL:GCK1

involved_in

GO:0043407: negative regulation of MAP kinase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00088246

P

Seeded From UniProt

complete

CAEEL:GCK1

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00088246

P

Seeded From UniProt

complete

CAEEL:MPK1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:H2L099

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.