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PMID:19825598

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Citation

Dyachok, J, Shao, MR, Vaughn, K, Bowling, A, Facette, M, Djakovic, S, Clark, L and Smith, L (2008) Plasma membrane-associated SCAR complex subunits promote cortical F-actin accumulation and normal growth characteristics in Arabidopsis roots. Mol Plant 1:990-1006

Abstract

The ARP2/3 complex, a highly conserved nucleator of F-actin polymerization, and its activator, the SCAR complex, have been shown to play important roles in leaf epidermal cell morphogenesis in Arabidopsis. However, the intracellular site(s) and function(s) of SCAR and ARP2/3 complex-dependent actin polymerization in plant cells remain unclear. We demonstrate that putative SCAR complex subunits BRK1 and SCAR1 are localized to the plasma membrane at sites of cell growth and wall deposition in expanding cells of leaves and roots. BRK1 localization is SCAR-dependent, providing further evidence of an association between these proteins in vivo. Consistent with plasma membrane localization of SCAR complex subunits, cortical F-actin accumulation in root tip cells is reduced in brk1 mutants. Moreover, mutations disrupting the SCAR or ARP2/3 complex reduce the growth rate of roots and their ability to penetrate semi-solid medium, suggesting reduced rigidity. Cell walls of mutant roots exhibit abnormal structure and composition at intercellular junctions where BRK1 and SCAR1 are enriched in the adjacent plasma membrane. Taken together, our results suggest that SCAR and ARP2/3 complex-dependent actin polymerization promotes processes at the plasma membrane that are important for normal growth and wall assembly.

Links

PubMed Online version:10.1093/mp/ssn059

Keywords

Actin-Related Protein 2-3 Complex/metabolism; Actins/metabolism; Arabidopsis/cytology; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis/ultrastructure; Arabidopsis Proteins/metabolism; Cell Membrane/metabolism; Cell Membrane/ultrastructure; Cell Wall/metabolism; Cell Wall/ultrastructure; Membrane Proteins/metabolism; Microsomes/metabolism; Microsomes/ultrastructure; Multiprotein Complexes/metabolism; Mutation/genetics; Plant Extracts/metabolism; Plant Roots/cytology; Plant Roots/growth & development; Plant Roots/metabolism; Plant Roots/ultrastructure; Protein Binding; Protein Subunits/metabolism; Recombinant Fusion Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:SCAR1

located_in

GO:0043229: intracellular organelle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:SCAR1

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:SCAR1

located_in

GO:0071944: cell periphery

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:BRK1

located_in

GO:0071944: cell periphery

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:BRK1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PINI

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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