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PMID:19822670

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Citation

Koto, A, Kuranaga, E and Miura, M (2009) Temporal regulation of Drosophila IAP1 determines caspase functions in sensory organ development. J. Cell Biol. 187:219-31

Abstract

The caspases comprise a family of cysteine proteases that function in various cellular processes, including apoptosis. However, how the balance is struck between the caspases' role in cell death and their nonapoptotic functions is unclear. To address this issue, we monitored the protein turnover of an endogenous caspase inhibitor, Drosophila IAP1 (DIAP1). DIAP1 is an E3 ubiquitin ligase that promotes the ubiquitination of caspases and thereby prevents caspase activation. For this study, we developed a fluorescent probe to monitor DIAP1 turnover in the external sensory organ precursor (SOP) lineage of living Drosophila. The SOP divides asymmetrically to make the shaft, socket, and sheath cells, and the neuron that comprise each sensory organ. We found that the quantity of DIAP1 changed dramatically depending on the cell type and maturity, and that the temporal regulation of DIAP1 turnover determines whether caspases function nonapoptotically in cellular morphogenesis or cause cell death.

Links

PubMed PMC2768825 Online version:10.1083/jcb.200905110

Keywords

Animals; Apoptosis; Caspases/metabolism; Cell Line; Cell Lineage; Cell Shape; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/genetics; Drosophila melanogaster/growth & development; Drosophila melanogaster/metabolism; Gene Expression Regulation, Developmental; Inhibitor of Apoptosis Proteins/genetics; Inhibitor of Apoptosis Proteins/metabolism; Sense Organs/cytology; Sense Organs/growth & development; Sense Organs/metabolism; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:DIAP1

involved_in

GO:0045035: sensory organ precursor cell division

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0086657

P

Seeded From UniProt

complete

DROME:Q7KJQ4

involved_in

GO:0045035: sensory organ precursor cell division

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0260635

P

Seeded From UniProt

complete

DROME:Q86PE7

involved_in

GO:0045035: sensory organ precursor cell division

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0260635

P

Seeded From UniProt

complete

DROME:Q9V3Y8

involved_in

GO:0045035: sensory organ precursor cell division

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0260635

P

Seeded From UniProt

complete

DROME:IAP1

involved_in

GO:0045035: sensory organ precursor cell division

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0086657

P

Seeded From UniProt

complete


See also

References

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