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PMID:19822128

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Citation

Hayashi, T, Ishimori, C, Takahashi-Niki, K, Taira, T, Kim, YC, Maita, H, Maita, C, Ariga, H and Iguchi-Ariga, SM (2009) DJ-1 binds to mitochondrial complex I and maintains its activity. Biochem. Biophys. Res. Commun. 390:667-72

Abstract

Parkinson's disease (PD) is caused by neuronal cell death, and oxidative stress and mitochondrial dysfunction are thought to be responsible for onset of PD. DJ-1, a causative gene product of a familial form of Parkinson's disease, PARK7, plays roles in transcriptional regulation and anti-oxidative stress. The possible mitochondrial function of DJ-1 has been proposed, but its exact function remains unclear. In this study, we found that DJ-1 directly bound to NDUFA4 and ND1, nuclear and mitochondrial DNA-encoding subunits of mitochondrial complex I, respectively, and was colocalized with complex I and that complex I activity was reduced in DJ-1-knockdown NIH3T3 and HEK293 cells. These findings suggest that DJ-1 is an integral mitochondrial protein and that DJ-1 plays a role in maintenance of mitochondrial complex I activity.

Links

PubMed Online version:10.1016/j.bbrc.2009.10.025

Keywords

Animals; Electron Transport Complex I/metabolism; Electron Transport Complex IV/genetics; Electron Transport Complex IV/metabolism; Gene Knockdown Techniques; Humans; Intracellular Signaling Peptides and Proteins/genetics; Intracellular Signaling Peptides and Proteins/metabolism; Mice; Oncogene Proteins/genetics; Oncogene Proteins/metabolism; Parkinson Disease/enzymology; Protein Binding

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:NDUA4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q99497

F

  • occurs_in:(UBERON:0000955)

Seeded From UniProt

complete

HUMAN:NDUS3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q99497

F

Seeded From UniProt

complete

HUMAN:NU1M

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q99497

F

  • occurs_in:(UBERON:0000955)

Seeded From UniProt

complete

HUMAN:PARK7

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O00483

F

Seeded From UniProt

complete

HUMAN:PARK7

enables

GO:0019899: enzyme binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P03886

F

  • occurs_in:(UBERON:0000955)

Seeded From UniProt

complete

HUMAN:PARK7

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PARK7

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PARK7

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PARK7

enables

GO:0044877: protein-containing complex binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PARK7

enables

GO:0019899: enzyme binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O75489

F

Seeded From UniProt

complete

HUMAN:PARK7

enables

GO:0019899: enzyme binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P03886

F

occurs_in:(UBERON:0000955)

Seeded From UniProt

complete

HUMAN:PARK7

colocalizes_with

GO:0005747: mitochondrial respiratory chain complex I

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PARK7

part_of

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PARK7

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PARK7

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PARK7

involved_in

GO:1902958: positive regulation of mitochondrial electron transport, NADH to ubiquinone

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:PARK7

involved_in

GO:1902958: positive regulation of mitochondrial electron transport, NADH to ubiquinone

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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