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PMID:19805279

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Citation

Mima, J and Wickner, W (2009) Phosphoinositides and SNARE chaperones synergistically assemble and remodel SNARE complexes for membrane fusion. Proc. Natl. Acad. Sci. U.S.A. 106:16191-6

Abstract

Yeast vacuole fusion requires 4 SNAREs, 2 SNARE chaperone systems (Sec17p/Sec18p/ATP and the HOPS complex), and 2 phosphoinositides, phosphatidylinositol 3-phosphate [PI(3)P] and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P(2)]. By reconstituting proteoliposomal fusion with purified components, we now show that phosphoinositides have 4 distinct roles: PI(3)P is recognized by the PX domain of the SNARE Vam7p; PI(3)P enhances the capacity of membrane-bound SNAREs to drive fusion in the absence of SNARE chaperones; either PI(3)P or PI(4,5)P(2) can activate SNARE chaperones for the recruitment of Vam7p into fusion-competent SNARE complexes; and either PI(3)P or PI(4,5)P(2) strikingly promotes synergistic SNARE complex remodeling by Sec17p/Sec18p/ATP and HOPS. This ternary synergy of phosphoinositides and 2 SNARE chaperone systems is required for rapid fusion.

Links

PubMed PMC2752541 Online version:10.1073/pnas.0908694106

Keywords

Adenosine Triphosphatases/metabolism; Adenosine Triphosphate/metabolism; Electrophoresis, Polyacrylamide Gel; Immunoblotting; Macromolecular Substances/metabolism; Membrane Fusion; Membrane Proteins/metabolism; Molecular Chaperones/metabolism; Phosphatidylinositol Phosphates/metabolism; Phosphatidylinositols/metabolism; Protein Binding; Protein Transport; Qc-SNARE Proteins/metabolism; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins/metabolism; Synaptosomal-Associated Protein 25; Vacuoles/metabolism; Vesicular Transport Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:VAM7

enables

GO:0005484: SNAP receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:VTI1

enables

GO:0005484: SNAP receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:VAM3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q12255

F

Seeded From UniProt

complete

YEAST:NYV1

enables

GO:0005484: SNAP receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:NYV1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q12241

F

Seeded From UniProt

complete


See also

References

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