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PMID:19799893

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Citation

Pellegrino, MW, Gasser, RB, Sprenger, F, Stetak, A and Hajnal, A (2009) The conserved zinc finger protein VAB-23 is an essential regulator of epidermal morphogenesis in Caenorhabditis elegans. Dev. Biol. 336:84-93

Abstract

Caenorhabditis elegans is an excellent model to observe cell movements and shape changes during the morphogenesis of the egg-shaped embryo into an elongated tube-like larva. Although much is known about the structural determinants involved in epidermal morphogenesis, relatively little is known about the transcriptional and post-transcriptional regulatory networks involved. Here, we describe the identification and functional characterization of the novel nuclear protein VAB-23, which belongs to a conserved protein family found in all metazoans. C. elegans VAB-23 is essential for ventral closure and elongation of the embryo. Time-lapse analysis indicates that VAB-23 is required for the formation of proper cell contacts between contralateral pairs of ventral epidermal cells. Tissue-specific rescue experiments reveal a function of VAB-23 in ventral neuroblasts that control the enclosure of the embryo by the overlaying epidermal cells. Finally, we provide evidence suggesting a role of VAB-23 in post-transcriptional gene regulation. We thus propose that VAB-23 regulates the expression of multiple secreted guidance cues in ventral neuroblasts that direct the migration of the overlaying epidermal cells. Members of the VAB-23 family may perform similar functions during morphogenesis in other metazoans.

Links

PubMed Online version:10.1016/j.ydbio.2009.09.036

Keywords

Amino Acid Sequence; Animals; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans Proteins/physiology; Carrier Proteins/genetics; Carrier Proteins/metabolism; Carrier Proteins/physiology; Conserved Sequence; Embryo, Nonmammalian/embryology; Embryo, Nonmammalian/metabolism; Epidermis/embryology; Epidermis/metabolism; Gene Expression Regulation, Developmental; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Immunohistochemistry; Microscopy, Confocal; Molecular Sequence Data; Morphogenesis; Nuclear Proteins/classification; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Zinc Fingers

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:Q23664

involved_in

GO:0010172: embryonic body morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00250906

P

Seeded From UniProt

complete

CAEEL:Q23664

located_in

GO:0016607: nuclear speck

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q23664

involved_in

GO:0048730: epidermis morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00250906

P

Seeded From UniProt

complete

CAEEL:Q23664

involved_in

GO:0098609: cell-cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00250906

P

Seeded From UniProt

complete

CAEEL:Q23664

involved_in

GO:0040025: vulval development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:Q65ZB3

involved_in

GO:0010172: embryonic body morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00250906

P

Seeded From UniProt

complete

CAEEL:Q65ZB3

located_in

GO:0016607: nuclear speck

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q65ZB3

involved_in

GO:0048730: epidermis morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00250906

P

Seeded From UniProt

complete

CAEEL:Q65ZB3

involved_in

GO:0098609: cell-cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00250906

P

Seeded From UniProt

complete

CAEEL:Q65ZB3

involved_in

GO:0040025: vulval development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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