GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:1979948

From GONUTS
Jump to: navigation, search
Citation

Moorman, AF, de Boer, PA, Charles, R and Lamers, WH (1990) Diet- and hormone-induced reversal of the carbamoylphosphate synthetase mRNA gradient in the rat liver lobulus. FEBS Lett. 276:9-13

Abstract

A hybridocytochemical analysis of adult liver from normal control and from hormonally and dietary-treated rats was carried out, using radioactively-labelled probes for the mRNAs of glutamine synthetase (GS), carbamoylphosphate synthetase (CPS) and phosphoenolpyruvate carboxykinase (PEPCK). In line with previous findings, GS mRNA is exclusively expressed in a small pericentral compartment, CPS mRNA exclusively in a contiguous large periportal compartment and PEPCK mRNA across the entire porto-central distance. The density of labelling in CPS and PEPCK mRNA-positive hepatocytes decreases in a porto-central direction. Starvation resulted in a reversal of the gradient of CPS mRNA within its periportal compartment; glucose refeeding counteracted this effect. Livers of glucocorticosteroid-treated, starved or diabetic rats also revealed a reversal of the normal gradient of CPS mRNA, but now across the entire porto-central distance. The patterns of expression of GS and PEPCK mRNA remained essentially unchanged, notwithstanding substantial changes in the levels of expression. It is concluded that blood-borne factors constitute the major determinants for the expression patterns of CPS mRNA within the context of the architecture of the liver lobulus.

Links

PubMed

Keywords

Animals; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/genetics; DNA Probes; Dexamethasone/pharmacology; Diabetes Mellitus, Experimental/enzymology; Glutamate-Ammonia Ligase/genetics; Liver/drug effects; Liver/enzymology; Organ Specificity; Phosphoenolpyruvate Carboxykinase (GTP)/genetics; RNA, Messenger/drug effects; RNA, Messenger/genetics; RNA, Messenger/metabolism; Rats; Rats, Inbred Strains; Reference Values

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.