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PMID:19788910

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Citation

Fujimura, L, Watanabe-Takano, H, Sato, Y, Tokuhisa, T and Hatano, M (2009) Prickle promotes neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. Neurosci. Lett. 467:6-10

Abstract

Murine Prickle1 and Prickle2 belong to the planar cell polarity genes. Prickle2 but not Prickle1 gene expression was induced in C1300 neuroblastoma cells during neurite-like process formation induced by retinoic acid (RA). Over-expression of Prickle1 or Prickle2 in C1300 cells induced striking neurite-like process formation in the absence of RA. Since Prickle binds to Dishevelled (Dvl) to induce its degradation in Drosophila, we examined the participation of Dvl protein in the neurite-like process formation of C1300 cells. Upon induction of the neurite-like process formation, the amount of Dvl1 protein decreased. Prickle1 and Prickle2 could associate with Dvl1 and over-expression of Prickle1 or Prickle2 resulted in the reduction of Dvl1 protein in C1300 cells. Furthermore, over-expression of Dvl1 in C1300 cells prevented the neurite-like process formation induced by Prickle1 or Prickle2 over-expression. Thus, Prickle1 and Prickle2 promote neurite-like process formation of C1300 cells via the Dvl1 dependent mechanism.

Links

PubMed Online version:10.1016/j.neulet.2009.09.050

Keywords

Adaptor Proteins, Signal Transducing/metabolism; Animals; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Differentiation/physiology; Cell Line; Cell Line, Tumor; Cells, Cultured; Cerebellum/physiology; Humans; LIM Domain Proteins; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurites/physiology; Neurons/physiology; Phosphoproteins/metabolism; RNA, Messenger; Signal Transduction; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:DVL1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q3U5C7
PR:Q80Y24

F

Seeded From UniProt

complete

MOUSE:PRIC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P51141

F

Seeded From UniProt

complete

MOUSE:PRIC2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P51141

F

Seeded From UniProt

complete

MOUSE:PRIC2

acts_upstream_of_or_within

GO:0031175: neuron projection development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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