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PMID:19765229

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Citation

Lim, GH, Zhang, X, Chung, MS, Lee, DJ, Woo, YM, Cheong, HS and Kim, CS (2010) A putative novel transcription factor, AtSKIP, is involved in abscisic acid signalling and confers salt and osmotic tolerance in Arabidopsis. New Phytol. 185:103-13

Abstract

We identified and functionally characterized the AtSKIP gene (At1g77180), an Arabidopsis homologue of SNW/SKIP, under abiotic stresses. Although the SNW/SKIP protein has been implicated as a critical transcription cofactor, its biological functions have yet to be reported in any plant. Recently, we have isolated Salt-tolerance genes (SATs) via the overexpression screening of yeast with a maize cDNA library. One of the selected genes (SAT2) appeared to confer elevated tolerance to salt. Maize SAT2 cDNA encodes a homologue of the human SNW/SKIP transcriptional coregulator. Treatment with salt, mannitol and abscisic acid induced AtSKIP expression. Ectopic expression of the AtSKIP gene modulated the induction of salt tolerance, dehydration resistance and insensitivity towards abscisic acid under stress conditions. By contrast, atskip antisense lines displayed reduced tolerance to abiotic stresses during germination. Moreover, a decrease in AtSKIP expression resulted in an abnormal phenotype. We further determined that the AtSKIP protein activated the transcription of a reporter gene in yeast. Green fluorescent protein-tagged AtSKIP was localized in the nuclei of both onion cells and transgenic Arabidopsis cells. Taken together, these results suggest that AtSKIP functions as both a positive regulator and putative potential transcription factor in the abiotic stress signalling pathway.

Links

PubMed Online version:10.1111/j.1469-8137.2009.03032.x

Keywords

Abscisic Acid/genetics; Abscisic Acid/physiology; Acetyltransferases/genetics; Adaptation, Physiological/genetics; Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; DNA, Complementary; Dehydration; Gene Expression Regulation, Plant; Gene Library; Genes, Plant; Genes, Reporter; Germination; Humans; Mannitol; Mutation; Onions; Osmosis; Phenotype; Plants, Genetically Modified; Salt-Tolerance/genetics; Signal Transduction/genetics; Sodium Chloride; Stress, Physiological/genetics; Transcription Factors/genetics; Transcription Factors/metabolism; Yeasts/genetics; Zea mays/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:SKIP

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:SKIP

acts_upstream_of_or_within

GO:0010555: response to mannitol

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SKIP

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SKIP

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SKIP

acts_upstream_of_or_within

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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