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PMID:19732381

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Citation

Zhang, M, Henquet, M, Chen, Z, Zhang, H, Zhang, Y, Ren, X, van der Krol, S, Gonneau, M, Bosch, D and Gong, Z (2009) LEW3, encoding a putative alpha-1,2-mannosyltransferase (ALG11) in N-linked glycoprotein, plays vital roles in cell-wall biosynthesis and the abiotic stress response in Arabidopsis thaliana. Plant J. 60:983-99

Abstract

N-linked glycosylation is an essential protein modification that helps protein folding, trafficking and translocation in eukaryotic systems. The initial process for N-linked glycosylation shares a common pathway with assembly of a dolichol-linked core oligosaccharide. Here we characterize a new Arabidopsis thaliana mutant lew3 (leaf wilting 3), which has a defect in an alpha-1,2-mannosyltransferase, a homolog of ALG11 in yeast, that transfers mannose to the dolichol-linked core oligosaccharide in the last two steps on the cytosolic face of the ER in N-glycan precursor synthesis. LEW3 is localized to the ER membrane and expressed throughout the plant. Mutation of LEW3 caused low-level accumulation of Man(3)GlcNAc(2) and Man(4)GlcNAc(2) glycans, structures that are seldom detected in wild-type plants. In addition, the lew3 mutant has low levels of normal high-mannose-type glycans, but increased levels of complex-type glycans. The lew3 mutant showed abnormal developmental phenotypes, reduced fertility, impaired cellulose synthesis, abnormal primary cell walls, and xylem collapse due to disturbance of the secondary cell walls. lew3 mutants were more sensitive to osmotic stress and abscisic acid (ABA) treatment. Protein N-glycosylation was reduced and the unfolded protein response was more activated by osmotic stress and ABA treatment in the lew3 mutant than in the wild-type. These results demonstrate that protein N-glycosylation plays crucial roles in plant development and the response to abiotic stresses.

Links

PubMed Online version:10.1111/j.1365-313X.2009.04013.x

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Wall/metabolism; Cellulose/biosynthesis; Cloning, Molecular; Gene Expression Regulation, Plant; Genes, Essential; Genetic Complementation Test; Glycoproteins/genetics; Glycoproteins/metabolism; Glycosylation; Mannosyltransferases/genetics; Mannosyltransferases/metabolism; Molecular Sequence Data; Plant Transpiration; Promoter Regions, Genetic; RNA, Plant/genetics; Sequence Homology, Amino Acid; Sodium Chloride/pharmacology; Stress, Physiological; Unfolded Protein Response; Xylem/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ALG11

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:ALG11

involved_in

GO:0006490: oligosaccharide-lipid intermediate biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ALG11

enables

GO:0004377: GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ALG11

acts_upstream_of_or_within

GO:0006970: response to osmotic stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ALG11

acts_upstream_of_or_within

GO:0006487: protein N-linked glycosylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ALG11

located_in

GO:0005789: endoplasmic reticulum membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:ALG11

acts_upstream_of_or_within

GO:0009832: plant-type cell wall biogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ALG11

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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