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PMID:19706470

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Citation

Suloway, CJ, Chartron, JW, Zaslaver, M and Clemons, WM Jr (2009) Model for eukaryotic tail-anchored protein binding based on the structure of Get3. Proc. Natl. Acad. Sci. U.S.A. 106:14849-54

Abstract

The Get3 ATPase directs the delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER). TA-proteins are characterized by having a single transmembrane helix (TM) at their extreme C terminus and include many essential proteins, such as SNAREs, apoptosis factors, and protein translocation components. These proteins cannot follow the SRP-dependent co-translational pathway that typifies most integral membrane proteins; instead, post-translationally, these proteins are recognized and bound by Get3 then delivered to the ER in the ATP dependent Get pathway. To elucidate a molecular mechanism for TA protein binding by Get3 we have determined three crystal structures in apo and ADP forms from Saccharomyces cerevisae (ScGet3-apo) and Aspergillus fumigatus (AfGet3-apo and AfGet3-ADP). Using structural information, we generated mutants to confirm important interfaces and essential residues. These results point to a model of how Get3 couples ATP hydrolysis to the binding and release of TA-proteins.

Links

PubMed PMC2736419 Online version:10.1073/pnas.0907522106

Keywords

Adenosine Triphosphatases/chemistry; Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Aspergillus fumigatus/enzymology; Crystallography, X-Ray; Guanine Nucleotide Exchange Factors/chemistry; Guanine Nucleotide Exchange Factors/genetics; Guanine Nucleotide Exchange Factors/metabolism; Membrane Fusion Proteins/chemistry; Membrane Fusion Proteins/metabolism; Models, Molecular; Nucleic Acid Conformation; Nucleotides/chemistry; Nucleotides/metabolism; Phenotype; Protein Binding; Protein Structure, Quaternary; Protein Structure, Tertiary; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Structural Homology, Protein

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ASPFU:GET3

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q4WY07

F

Seeded From UniProt

complete

YEAST:GET3

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q12154

F

Seeded From UniProt

complete

Notes

See also

References

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