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PMID:19700618

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Citation

Arrington, CB and Yost, HJ (2009) Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo. Development 136:3143-52

Abstract

One of the first steps in zebrafish heart and gut organogenesis is the migration of bilateral primordia to the midline to form cardiac and gut tubes. The mechanisms that regulate this process are poorly understood. Here we show that the proteoglycan syndecan 2 (Sdc2) expressed in the extra-embryonic yolk syncytial layer (YSL) acts locally at the YSL-embryo interface to direct organ primordia migration, and is required for fibronectin and laminin matrix assembly throughout the embryo. Surprisingly, neither endogenous nor exogenous sdc2 expressed in embryonic cells can compensate for knockdown of sdc2 in the YSL, indicating that Sdc2 expressed in extra-embryonic tissues is functionally distinct from Sdc2 in embryonic cells. The effects of sdc2 knockdown in the YSL can be rescued by extra-embryonic Sdc2 lacking an extracellular proteolytic cleavage (shedding) site, but not by extra-embryonic Sdc2 lacking extracellular glycosaminoglycan (GAG) addition sites, suggesting that distinct GAG chains on extra-embryonic Sdc2 regulate extracellular matrix assembly, cell migration and epithelial morphogenesis of multiple organ systems throughout the embryo.

Links

PubMed PMC2730369 Online version:10.1242/dev.031492

Keywords

Animals; Cell Lineage; Cell Movement/physiology; Cell Polarity; Egg Yolk/metabolism; Embryo, Nonmammalian/anatomy & histology; Embryo, Nonmammalian/physiology; Endocardium/cytology; Endocardium/metabolism; Endoderm/cytology; Endoderm/metabolism; Gene Knockdown Techniques; Membrane Proteins/genetics; Membrane Proteins/metabolism; Morphogenesis/physiology; Myocytes, Cardiac/cytology; Myocytes, Cardiac/metabolism; Syndecan-2/genetics; Syndecan-2/metabolism; Zebrafish/anatomy & histology; Zebrafish/embryology; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:Q66I66

involved_in

GO:0060973: cell migration involved in heart development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-2

P

Seeded From UniProt

complete

DANRE:Q66I66

involved_in

GO:0060973: cell migration involved in heart development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-1

P

Seeded From UniProt

complete

DANRE:Q66I66

involved_in

GO:0048565: digestive tract development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-2

P

Seeded From UniProt

complete

DANRE:Q66I66

involved_in

GO:0048565: digestive tract development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-1

P

Seeded From UniProt

complete

DANRE:Q66I66

involved_in

GO:0097435: supramolecular fiber organization

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-2

P

Seeded From UniProt

complete

DANRE:Q66I66

involved_in

GO:0097435: supramolecular fiber organization

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-1

P

Seeded From UniProt

complete

DANRE:Q90ZY9

involved_in

GO:0060973: cell migration involved in heart development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-2

P

Seeded From UniProt

complete

DANRE:Q90ZY9

involved_in

GO:0060973: cell migration involved in heart development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-1

P

Seeded From UniProt

complete

DANRE:Q90ZY9

involved_in

GO:0048565: digestive tract development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-2

P

Seeded From UniProt

complete

DANRE:Q90ZY9

involved_in

GO:0048565: digestive tract development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-1

P

Seeded From UniProt

complete

DANRE:Q90ZY9

involved_in

GO:0097435: supramolecular fiber organization

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-2

P

Seeded From UniProt

complete

DANRE:Q90ZY9

involved_in

GO:0097435: supramolecular fiber organization

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100513-1

P

Seeded From UniProt

complete


See also

References

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