GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19682286

From GONUTS
Jump to: navigation, search
Citation

Huang, L, Yang, S, Zhang, S, Liu, M, Lai, J, Qi, Y, Shi, S, Wang, J, Wang, Y, Xie, Q and Yang, C (2009) The Arabidopsis SUMO E3 ligase AtMMS21, a homologue of NSE2/MMS21, regulates cell proliferation in the root. Plant J. 60:666-78

Abstract

hMMS21 is a SUMO E3 ligase required for the prevention of DNA damage-induced apoptosis, and acts by facilitating DNA repair in human cells. The Arabidopsis genome contains a putative MMS21 homologue capable of interacting with the SUMO E2 conjugating enzyme AtSCE1a, as indicated by a yeast two-hybrid screen and bimolecular fluorescence complementation experiments. In vitro and in vivo data demonstrated that AtMMS21 was a SUMO E3 ligase. We identified the Arabidopsis AtMMS21 null T-DNA insertion mutant mms21-1, which had a short-root phenotype, and affected cell proliferation in the apical root meristem, as indicated by impaired expression of the cell division marker CYCB1:GUS in mms21-1 roots. The mms21-1 roots had reduced responses to exogenous cytokinins, and decreased expression of the cytokinin-induced genes ARR3, ARR4, ARR5 and ARR7, compared with the wild type. Thus, our findings suggest that the AtMMS21 gene is involved in root development via cell-cycle regulation and cytokinin signalling.

Links

PubMed Online version:10.1111/j.1365-313X.2009.03992.x

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Proliferation; Cytokinins/pharmacology; Gene Expression Regulation, Plant; Molecular Sequence Data; Mutagenesis, Insertional; Plant Roots/cytology; Plant Roots/enzymology; Plant Roots/genetics; Plant Roots/growth & development; RNA, Plant/genetics; Signal Transduction; Small Ubiquitin-Related Modifier Proteins/genetics; Small Ubiquitin-Related Modifier Proteins/metabolism; Ubiquitin-Protein Ligases/genetics; Ubiquitin-Protein Ligases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:SCE1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8GYH7

F

Seeded From UniProt

complete

ARATH:NSE2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q42551

F

Seeded From UniProt

complete

ARATH:NSE2

involved_in

GO:0016925: protein sumoylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NSE2

enables

GO:0019789: SUMO transferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:NSE2

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:NSE2

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NSE2

involved_in

GO:0010082: regulation of root meristem growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NSE2

involved_in

GO:0045931: positive regulation of mitotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NSE2

involved_in

GO:0080038: positive regulation of cytokinin-activated signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.