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PMID:19633175

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Citation

Liu, R, Abreu-Blanco, MT, Barry, KC, Linardopoulou, EV, Osborn, GE and Parkhurst, SM (2009) Wash functions downstream of Rho and links linear and branched actin nucleation factors. Development 136:2849-60

Abstract

Wiskott-Aldrich Syndrome (WAS) family proteins are Arp2/3 activators that mediate the branched-actin network formation required for cytoskeletal remodeling, intracellular transport and cell locomotion. Wasp and Scar/WAVE, the two founding members of the family, are regulated by the GTPases Cdc42 and Rac, respectively. By contrast, linear actin nucleators, such as Spire and formins, are regulated by the GTPase Rho. We recently identified a third WAS family member, called Wash, with Arp2/3-mediated actin nucleation activity. We show that Drosophila Wash interacts genetically with Arp2/3, and also functions downstream of Rho1 with Spire and the formin Cappuccino to control actin and microtubule dynamics during Drosophila oogenesis. Wash bundles and crosslinks F-actin and microtubules, is regulated by Rho1, Spire and Arp2/3, and is essential for actin cytoskeleton organization in the egg chamber. Our results establish Wash and Rho as regulators of both linear- and branched-actin networks, and suggest an Arp2/3-mediated mechanism for how cells might coordinately regulate these structures.

Links

PubMed PMC2730411 Online version:10.1242/dev.035246

Keywords

Actin-Related Protein 2-3 Complex/genetics; Actin-Related Protein 2-3 Complex/metabolism; Actins/genetics; Actins/metabolism; Animals; Cytoskeleton/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/anatomy & histology; Drosophila melanogaster/metabolism; Female; Mice; Mice, Inbred BALB C; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Microtubules/metabolism; Oogenesis/physiology; Ovary/cytology; Ovary/metabolism; Wiskott-Aldrich Syndrome Protein/genetics; Wiskott-Aldrich Syndrome Protein/metabolism; rho GTP-Binding Proteins/genetics; rho GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:A0A0B4KH08

involved_in

GO:0001578: microtubule bundle formation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:A0A0B4KH08

enables

GO:0051020: GTPase binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0010341

F

Seeded From UniProt

complete

DROME:F2FB81

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:H5V8D0

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:M9NCT2

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:M9PCP4

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CDC42

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0037750

F

Seeded From UniProt

complete

DROME:RHO1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0033692

F

Seeded From UniProt

complete

DROME:RHO1

involved_in

GO:0051493: regulation of cytoskeleton organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q0KI93

involved_in

GO:0001578: microtubule bundle formation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q0KI93

enables

GO:0051020: GTPase binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0010341

F

Seeded From UniProt

complete

DROME:WASH1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0003475

F

Seeded From UniProt

complete

DROME:WASH1

involved_in

GO:0001578: microtubule bundle formation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:WASH1

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:WASH1

involved_in

GO:0051764: actin crosslink formation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:WASH1

involved_in

GO:0051017: actin filament bundle assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:WASH1

involved_in

GO:0048477: oogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:WASH1

enables

GO:0051020: GTPase binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0014020

F

Seeded From UniProt

complete

DROME:Q86B74

involved_in

GO:0001578: microtubule bundle formation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q86B74

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8SYK0

involved_in

GO:0001578: microtubule bundle formation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8SYK0

enables

GO:0051020: GTPase binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0010341

F

Seeded From UniProt

complete

DROME:Q95SC7

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:SPIR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0033692

F

Seeded From UniProt

complete

DROME:SPIR

involved_in

GO:0051493: regulation of cytoskeleton organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VAT0

involved_in

GO:0001578: microtubule bundle formation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VAT0

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VH71

involved_in

GO:0001578: microtubule bundle formation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VH71

enables

GO:0051020: GTPase binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0010341

F

Seeded From UniProt

complete

DROME:Q9VKM2

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:X2J5L4

involved_in

GO:0034314: Arp2/3 complex-mediated actin nucleation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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