GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19631203

From GONUTS
Jump to: navigation, search
Citation

Blagden, SP, Gatt, MK, Archambault, V, Lada, K, Ichihara, K, Lilley, KS, Inoue, YH and Glover, DM (2009) Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development. Dev. Biol. 334:186-97

Abstract

As the influence of mRNA translation upon cell cycle regulation becomes clearer, we searched for genes that might specify such control in Drosophila. A maternal-effect lethal screen identified mutants in the Drosophila gene for Larp (La-related protein) which displayed maternal-effect lethality and male sterility. A role for La protein has already been implicated in mRNA translation whereas Larp has been proposed to regulate mRNA stability. Here we demonstrate that Larp exists in a physical complex with, and also interacts genetically with, the translation regulator poly(A)-binding protein (PABP). Most mutant alleles of pAbp are embryonic lethal. However hypomorphic pAbp alleles show similar meiotic defects to larp mutants. We find that larp mutant-derived syncytial embryos show a range of mitotic phenotypes, including failure of centrosomes to migrate around the nuclear envelope, detachment of centrosomes from spindle poles, the formation of multipolar spindle arrays and cytokinetic defects. We discuss why the syncytial mitotic cycles and male meiosis should have a particularly sensitive requirement for Larp proteins in regulating not only transcript stability but also potentially the translation of mRNAs.

Links

PubMed Online version:10.1016/j.ydbio.2009.07.016

Keywords

Alleles; Animals; Cytoplasm/metabolism; Drosophila/embryology; Drosophila/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Embryo, Nonmammalian/metabolism; Embryonic Development/physiology; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Larva/metabolism; Male; Meiosis; Mutation; Oocytes/metabolism; Poly(A)-Binding Proteins/metabolism; RNA Interference; RNA Stability; RNA, Messenger/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:PABP

involved_in

GO:0007140: male meiotic nuclear division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:LARP

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:LARP

involved_in

GO:0007140: male meiotic nuclear division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:LARP

involved_in

GO:0035186: syncytial blastoderm mitotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.