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PMID:19619494

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Citation

Okamoto, K, Kondo-Okamoto, N and Ohsumi, Y (2009) Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy. Dev. Cell 17:87-97

Abstract

Mitochondria are essential organelles that produce most of the energy for a cell, but concomitantly accumulate oxidative damage. Degradation of damaged mitochondria is critical for cell homeostasis, and this process is thought to be mediated by mitophagy, an autophagy-related pathway specific for mitochondria. However, whether mitochondria are selectively degraded, and how the autophagic machinery is targeted to mitochondria, remain largely unknown. Here we demonstrate that, in post-log phase cells under respiratory conditions, a substantial fraction of mitochondria are exclusively sequestered as cargoes and transported to the vacuole, a lytic compartment in yeast, in an autophagy-dependent manner. Interestingly, we found Atg32, a mitochondria-anchored protein essential for mitophagy that is induced during respiratory growth. In addition, our data suggest that Atg32 interacts with Atg8 and Atg11, autophagy-related proteins critical for recognition of cargo receptors. We propose that Atg32 acts as a mitophagy-specific receptor and regulates selective degradation of mitochondria.

Links

PubMed Online version:10.1016/j.devcel.2009.06.013

Keywords

Amino Acid Sequence; Autophagy/physiology; Cell Respiration/physiology; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Mitochondria/metabolism; Mitochondria/ultrastructure; Mitochondrial Membranes/metabolism; Mitochondrial Membranes/ultrastructure; Molecular Sequence Data; Receptors, Cytoplasmic and Nuclear/genetics; Receptors, Cytoplasmic and Nuclear/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Alignment; Two-Hybrid System Techniques; Vesicular Transport Proteins/genetics; Vesicular Transport Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:ATG32

involved_in

GO:0000422: autophagy of mitochondrion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:ATG32

located_in

GO:0031307: integral component of mitochondrial outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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